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Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultra-Broad Dynamic Range Colleen N. Loynachan, Michael R Thomas, Eleanor R Gray, Daniel A Richards, Jeongyun Kim, Benjamin S Miller, Jennifer Clare Brookes, Shweta Agarwal, Vijay Chudasama, Rachel A. McKendry, and Molly M. Stevens ACS Nano, Just Accepted Manuscript • DOI: 10.1021/ acsnano.7b06229 • Publication Date (Web): 07 Dec 2017 Downloaded from http://pubs.acs.org on December 7, 2017
Just Accepted
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ACS Nano
1 2 3 4 Patient 5 Plasma 6 7 8 Lyophilized 9 Pre-catalysis Conjugates 10 11 12 13 Antibody-PtNC 14 conjugates 15 16 17b Nanobody-cysteine 18 mutation 19 20 21 22 23 24 BromopyridazinedionePEG8-biotin 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
Colorless CN/DAB + H2O2
(i)
H2O2
Black Precipitate Oxidized CN/DAB
2 •OH
(ii)
Amplified lateral flow assay
Nanobodybiotin
Target analyte
Polystreptavidin
c Ultra-broad dynamic range of PtNC LFIA Catalytic amplification
PtNC LFIA
+
ELISA 4th gen. LFIA 10-1 100
ACS Paragon Plus Environment
101 102 103 104 [Analyte] pg·mL-1
a
Pt4+
b ACS Nano
Pt4+
Au Pt4+ 1 2 Pt4+ 3PVP + 4 L-Ascorbic Acid 5 6 65 ℃ 7 8 9 PtNC 10 11 12 c 13 i14 15 16 17 18 19 20 21 22 100 nm 23 24 0.18 nm iii 25 26 27 0.22 nm 2 nm 28 29 30 31 32 33 50 nm 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
d ii
iii iii
100 nm iv iv
e 311 220 200 111
ACS Paragon Plus Environment
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ACS Paragon Plus Environment
a
1 2 3 4 5 6 7 8 9 10 11 12 b 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 c 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
Post-development
ACS Nano
Pre-development
> 125
Free p24
Seropositive
> 125 99.7
pg·mL-1
21.9
