temperatures the isobutyraldehyde peak was not separated from the acetone peak. The concentrations of the various oxygenates in the composite sample used to produce this chromatogram were gram mole of the order of low3to per liter. The procedure of direct injection of the bisulfite solution into the GLC unit provides a rapid qualitative analysis. After a confirmed GLC qualitative analysis has been established, the procedure of direct solution injection could provide a basis for studies in quantitative analysis. This procedure in its present development, however, can provide a means for ascertaining the relative amounts of the oxygenates. The chromatograms shown in Figure 4, based on a trapping procedure, are subject to the effects of distortion of the relative amounts of the oxygenates
caused by varying partial condensations which most likely occur in the cold trapping needle. Figure 7 shows two chromatograms obtained by the injection of bag-trapped effluents from the preparatory GLC unit. One chromatogram (top panel) was derived from the cycle mode of engine operation and the other from the 40-m.p.h. cruise mode. At 45’ C., good resolution was obtained with the 20-foot Carbowax-Teflon column at 55 ml. per minute helium flow. The qualitative identity of the oxygenates for the two modes of engine operation is noted. This method, in contrast to direct solution injection, has the advantage of permitting an unmodified aliquot of the preparatory column effluent to be charged to a GLC unit operating a t lower temperatures where greater resolution can be obtained.
LITERATURE CITED
(1) Altshuller, A. P., Cohen, I. R., ANAL.
CHEY.33, 1180 (1961).
( 2 ) Altshuller, A. P., Leng, L. J., Ibid., 35, 1541 (1963).
(3) ~, Altshuller. A. P.. Miller. D. L.. Sleva. S . F., Ibid.,’33, 621 (1961j. ( 4 ) Barber, E. D., Lodge, J. P., Jr., Ibid., 35, 348 (1963). (5) Cohen, 1. R., Altshuller, A. P., Ibid., 33, 726 (1961). (6) Ellis, C. F., BuMines Rept. of Investinations 5822. 35 DD.. 1961. (7) Hughes, K. Ji’Hur
