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Protective Effect of Panax notoginseng Saponins on Acute Ethanol-induced Liver Injury is Associated with Ameliorating Hepatic Lipid Accumulation and Reducing Ethanol-mediated Oxidative Stress Ren-Bo Ding, Ke Tian, Yi-Wei Cao, Jiao-Lin Bao, Meng Wang, Chengwei He, Yuanjia Hu, Huanxing Su, and Jian-Bo Wan J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/jf502990n • Publication Date (Web): 10 Feb 2015 Downloaded from http://pubs.acs.org on February 13, 2015
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
TOC Graphic
Free Fatty acid
TG
Lipid accumulation
TG MDA SOD
Oxidative stress
ROS
CYP2E1
GSH
The potential targets of the inhibitory effects of PNS
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Journal of Agricultural and Food Chemistry
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Protective Effect of Panax notoginseng Saponins on Acute Ethanol-induced Liver
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Injury is Associated with Ameliorating Hepatic Lipid Accumulation and
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Reducing Ethanol-mediated Oxidative Stress
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Ren-Bo Dinga,1, Ke Tianb,1,Yi-Wei Caoa, Jiao-Lin Baoa, Meng Wanga, Chengwei Hea, Yuanjia Hua,
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Huanxing Sua, Jian-Bo Wana,*
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a
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China
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b
Center for Intestinal and Liver Inflammation Research, Stanley Manne Children's Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
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Ren-Bo Ding and Ke Tian contributed equally to this work.
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*Correspondence
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Dr. Jian-Bo Wan,
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Institute of Chinese Medical Sciences,
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University of Macau, Taipa, Macao, China.
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Tel: +853-397 4873; Fax: +853-28841 358
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E-mail:
[email protected] 23 1
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Journal of Agricultural and Food Chemistry
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Abstract
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The aim of present study was to evaluate the effects of Panax notoginseng saponins (PNS) against
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acute ethanol-induced liver injury and further to elucidate its probable mechanisms. Mice were
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treated with PNS (100 or 300 mg/kg) once daily for 7 consecutive days priors to ethanol gavage (4.7
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g/kg) every 12 h for a total of three doses. Acute alcohol gavage dramatically significantly increased
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serum activities of ALT (23.4 ± 5.0 IU/L vs. 11.7 ± 4.1 IU/L) and AST (52.6 ± 14.9 IU/L vs. 31.1 ±
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12.9 IU/L), and hepatic triglyceride level (4.04 ± 0.64 mg/g vs 1.92 ± 0.34 mg/g), these elevations
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were significantly diminished by pre-treatment with PNS at dose of 100 mg/kg or 300 mg/kg.
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Alcohol exposure markedly induced the lipolysis of white adipose tissue (WAT), up-regulated protein
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expression of the phosphorylated hormone-sensitive lipase (p-HSL, p
