RECENT ADVANCES in HISTOLOGICAL CHEMISTRY by the LINDERSTROM-LANG HOLTER TECHNIC and DEVELOPMENT of ULTRAMICRO TECHNICS* DAVID GLICK Laboratories of the Newark Beth
Israel Hospital, Newark, New
Jersey
HREE years ago the author reviewed for THIS pleasant atmosphere of the Danish laboratory. The methods were developed as their need arose in particuzyme studies in histological chemistry by the lar cases, and at the present time there is a wide variety Linderstrfim-Lang Holter technic (I). In recent years of these methods available for studies of enzymes and the technic designed by Linderstrfim-Lang and Holter other biologically important constituents in histologically defined samples of tissue. It is the purpose of the present communication, in part, to outline the new procedures and summarize their applications so that the picture given previously may he brought up-to-date. It may be recalled-that the methods developed for the histochemical studies were thousand-fold r e h e ments of accepted macro procedures, refinements which permitted investigations of single microtome sections of tissue or single cells of the larger type, such as ova of marine invertebrates. However, in order to enable studies of single cells, and parts of cells, an approximate hundred-thousand-fold rehement of macro procedures would be required. It is questionable whether this goal can be conveniently achieved by further development of the original titration apparatus, though the possibility remains. A buret ten times h e r than those they normally employ (hence graduated in increments of 0.02 cmm.) was designed by Linderstr@m-Langand Holter; the buret tube was of smaller bore, and the capillary forces operating in this tube made consistently accurate titrations impossible. Because of the mechanical limitations to further buret refinement, it was considered desirable to seek an essentially different method of approach for analysis of an ultramicro order. Within the last year Linderstr$m-Lang has developed two separate ultramicro technics which are based on different principles, and which, to a great extent, have diierent limitations so that one can often be used in cases unsuited to the other. a t the Carlsberg Laboratory in Copenhagen has been They possess possibilities of opening extensive fields of expanded considerably by them and their collabora- investigation not only in the histochemical, but in the tors, who come from many parts of the world to learn cytochemical realm as well. The new technics will be and to help further the histochemical studies in the described later in this paper.
T
JOURNAL the methods and applications of en-
* It waserroneously stated in the previous paper ( I ) that the
apparatus was available irom the Burrell Technical Supply Comoanv. Fifth Avcnuc. Pittsburch. Ymnsvlvania. The amarntus. h & & n g the new uitramicro &&men
