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Biochemistry 2010, 49, 2454–2463 DOI: 10.1021/bi1001085
Relationships among Visual Cycle Retinoids, Rhodopsin Phosphorylation, and Phototransduction in Mouse Eyes during Light and Dark Adaptation† Kimberly A. Lee,‡ Maria Nawrot,§ Gregory G. Garwin,§ John C. Saari,‡,§ and James B. Hurley*,‡ ‡
Department of Biochemistry (Box 357350) and §Department of Ophthalmology (Box 356485), University of Washington, Seattle, Washington 98195 Received January 24, 2010; Revised Manuscript Received February 15, 2010 ABSTRACT: Phosphorylation and regeneration of rhodopsin, the prototypical G-protein-coupled receptor, each can influence light and dark adaptation. To evaluate their relative contributions, we quantified rhodopsin, retinoids, phosphorylation, and photosensitivity in mice during a 90 min illumination followed by dark adaptation. During illumination, all-trans-retinyl esters and, to a lesser extent, all-trans-retinal accumulate and reach the steady state in
