Anal. Chem. 1998, 70, 3940-3945
Role of Barium Ions in the Anion-Exchange Chromatographic Separation of Carbohydrates with Pulsed Amperometric Detection Tommaso R. I. Cataldi,*,† Cristiana Campa,† Giovanna Margiotta,† and Sabino A. Bufo‡
Dipartimento di Chimica and Dipartimento di Produzione Vegetale, Universita` degli Studi della Basilicata, Via N. Sauro, 85-85100 Potenza, Italy
Barium ion is shown here as an effective component of alkaline mobile phases for improving the separation of carbohydrates in anion-exchange chromatography with pulsed amperometric detection. We demonstrate that the introduction of barium ensures a complete removal of carbonate, making it possible to carry out a large number of separations without column reequilibration after each run. This is particularly helpful when diluted alkaline eluents (i.e.,
