26 A n Enzyme Immunoassay for the Detection of Ciguatoxin And Competitive Inhibition by Related Natural Polyether Toxins 1
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Υ.HOKAMA ,L. H.KIMURA ,M. A.ABAD ,L.YOKOCHI ,PAUL J. SCHEUER , M.NUKINA ,T.YASUMOTO ,D. G.BADEN ,and Y. SHIMIZU 2
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Department of Pathology, University of Hawaii, Manoa, Honolulu, HI 96822 Department of Chemistry, University of Hawaii, Manoa, Honolulu, HI 96822 Faculty of Agriculture, Tohoku University, Sendai, Japan Department of Biochemistry, School of Medicine, University of Miami, Miami, FL 33101 Department of Pharmacognosy, University of Rhode Island, Kingston, RI 02881
Downloaded by AUBURN UNIV on January 10, 2018 | http://pubs.acs.org Publication Date: September 19, 1984 | doi: 10.1021/bk-1984-0262.ch026
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An enzyme immunoassay (EIA) procedure for the detec tion of ciguatoxin (CTX) and the assessment of struc turally related polyether toxins from fish tissue is presented in this study. Ciguatoxin is measured directly from fish tissue with anti-CTX serum pre viously prepared in a sheep immunized with CTX-human serum albumin conjugate. Fourteen of the 15 fish tissues from clinically documented ciguatera poison ing cases gave EIA ratios of greater than 1.50, defined as positive, while one sample gave a 1.31 ratio, defined as borderline. A total of 76 Seriola dumerili consumed by individuals with no incidence of ciguatera poisoning gave a mean and standard deviation (X ±S.D.) ratio of 1.00 ± 0.02, defined as negative. Ten other samples of the same fish, also consumed by individuals without poisoning gave a ratio of 1.00 ± 0.22. The results of a prelimi nary survey of 2 species Ctenochaetus strigosus and Lutjanus kasmira, demonstrated the presences of CTX and CTX-like toxins in the locations associated with ciguatera poisoning in Hawaii. The percentages of potentially toxic fishes were 14.4% to 15.4% respec tively, for theC.strigosus and L. kasmira. The comparison between liver and flesh tissues of several species of fishes showed significantly (P
