Article pubs.acs.org/ac
Serum Creatinine Detection by a Conducting-Polymer-Based Electrochemical Sensor To Identify Allograft Dysfunction Fang Wei,*,†,□ Scott Cheng,‡ Yael Korin,§ Elaine F. Reed,§ David Gjertson,§ Chih-ming Ho,○ H. Albin Gritsch,∇ and Jeffrey Veale*,∇,□ †
School of Dentistry, Dental Research Institute, University of California, Los Angeles, California 90095-1668, United States Keck School of Medicine, Department of Family Medicine, University of Southern California, Los Angeles, California 90089, United States § UCLA Immunogenetics Center, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, California 90095-1672, United States ○ Mechanical and Aerospace Engineering Department Bioengineering Department University of California, Los Angeles, California 90095, United States ∇ Department of Urology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095-1738, United States ‡
ABSTRACT: Kidney transplant recipients who have abnormally high creatinine levels in their blood often have allograft dysfunction secondary to rejection. Creatinine has become the preferred marker for renal dysfunction and is readily available in hospital clinical settings. We developed a rapid and accurate polymer-based electrochemical point-of-care (POC) assay for creatinine detection from whole blood to identify allograft dysfunction. The creatinine concentrations of 19 blood samples from transplant recipients were measured directly from clinical serum samples by the conducting polymer-based electrochemical (EC) sensor arrays. These measurements were compared to the traditional clinical laboratory assay. The time required for detection was
