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Bioactive Constituents, Metabolites, and Functions
Sodium butyrate supplementation alleviates the adaptive response to inflammation and modulates fatty acid metabolism in lipopolysaccharide-stimulated bovine hepatocytes Tianle Xu, Nana Ma, Yan Wang, Xiaoli Shi, Guangjun Chang, Juan J. Loor, and Xiangzhen Shen J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b01439 • Publication Date (Web): 07 Jun 2018 Downloaded from http://pubs.acs.org on June 7, 2018
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Journal of Agricultural and Food Chemistry 1
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Sodium
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inflammation
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lipopolysaccharide-stimulated bovine hepatocytes
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Tianle Xu1, Nana Ma1, Yan Wang1, Xiaoli Shi2, Guangjun Chang1, Juan J Loor3 and
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Xiangzhen Shen1*
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1
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P. R. China
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2
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Nanjing, 210095, P. R. China
butyrate
supplementation and
modulates
alleviates fatty
the
adaptive
acid
response
metabolism
to in
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095,
College of Animal Science and Technology, Nanjing Agricultural University,
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3
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Illinois, Urbana
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*
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College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, P.
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R. China
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Tel.: +86-25-84395505, Fax: +86-25-84398669, E-mail:
[email protected] Department of Animal Sciences and Division of Nutritional Sciences, University of
Corresponding author: Xiangzhen Shen
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract
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This study aimed to evaluate whether SB attenuates the hepatic response to
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LPS-induced inflammation in bovine hepatocytes. Hepatocytes isolated from cows at
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~160 day in milk (DIM) were exposed to 0.5 mmol/L SB for 18 h as pretreatment.
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Cells pretreated with SB were used for SB group, and those subjected to 4 µg/mL LPS
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challenge for 6 h were used for LSB group. The LPS-challenged hepatocytes showed
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increases in TNF-α and IL-6 production in culture medium (37±11, P < 0.05); these
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increases were attenuated by pretreatment with SB in the LSB group (267±4, P
