Biochemistry 1988, 27, 240-245
240
Steady-State Kinetics of Thiocyanate Oxidation Catalyzed by Human Salivary Peroxidase? Kenneth M . Pruitt,*!f Britta Mansson-Rahemtulla,s David C. Baldone,f and Firoz Rahemtullae Department of Biochemistry and School of Dentistry, The University of Alabama at Birmingham, Birmingham, Alabama 35294 Received March 30, 1987; Revised Manuscript Received July 6, 1987 A steady-state kinetic analysis was made of thiocyanate (SCN-) oxidation catalyzed by human peroxidase (SPO) isolated from parotid saliva. For comparative purposes, bovine lactoperoxidase (LPO) was also studied. Both enzymes followed the classical Theorell-Chance mechanism under the initial conditions [H202]
