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Environmental Processes
Stopping AI-2 chatter by means of an indigenous bacterium (Acinetobacter sp. DKY-1): A new anti-biofouling strategy in an MBR for wastewater treatment Kibaek Lee, Yea-Won Kim, Seonki Lee, Sang Hyun Lee, Chang Hyun Nahm, Hyeokpil Kwon, PyungKyu Park, Kwang-Ho Choo, Ismail Koyuncu, Anja Drews, Chung-Hak Lee, and Jung-Kee Lee Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b05824 • Publication Date (Web): 01 May 2018 Downloaded from http://pubs.acs.org on May 2, 2018
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Environmental Science & Technology
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Stopping AI-2 chatter by means of an indigenous bacterium (Acinetobacter
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sp. DKY-1): A new anti-biofouling strategy in an MBR for wastewater
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treatment
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Kibaek Lee1, 5, Yea-Won Kim2, Seonki Lee1, Sang Hyun Lee1, Chang Hyun Nahm1, Hyeokpil Kwon1,
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Pyung-Kyu Park3, Kwang-Ho Choo4, Ismail Koyuncu6, Anja Drews7, Chung-Hak Lee1*, and Jung-
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Kee Lee2*
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School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Republic of Korea
Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon, 35345, Republic of Korea Department of Environmental Engineering, Yonsei University, Wonju, 26493, Republic of Korea Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea Advanced Institute of Water Industry, Kyungpook National University, Daegu, 41566, Republic of Korea Department of Environmental Engineering, Istanbul Technical University, Istanbul, 34469, Turkey
School of Life Science Engineering (Engineering II), HTW Berlin-University of Applied Sciences, Berlin, 12459, Germany
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*Correspondence to: Chung-Hak Lee, Tel: +82-10-8770-0672, E-mail:
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Jung-Kee Lee, Tel: +82-10-8737-9424, E-mail:
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Abstract
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Bacterial quorum quenching (QQ) by means of degrading signaling molecules has been applied to
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anti-biofouling strategy in a membrane bioreactor (MBR) for wastewater treatment. However, the
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target signaling molecules have been limited to N-acyl homoserine lactones participating in intra-
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species quorum sensing. Here, an approach to disrupt autoinducer-2 (AI-2) signaling molecules
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participating in inter-species quorum sensing, was pursued as a next-generation anti-biofouling 1
ACS Paragon Plus Environment
Environmental Science & Technology
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strategy in an MBR for wastewater treatment. We isolated an indigenous QQ bacterium
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(Acinetobacter sp. DKY-1) that can attenuate the expression of quorum sensing (QS) response
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through inactivation of autoinducer-2 signaling molecule, 4,5-dihydroxy-2,3-pentanedione (DPD)
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among four kinds of autoinducer-2 QS bacteria. DKY-1 released AI-2 QQ compound(s), which was
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verified to be hydrophilic with a molecular weight
