Strategy for Structural Elucidation of ... - ACS Publications

Subscriber access provided by IDAHO STATE UNIV

Article

A Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria Matthew J Amicucci, Ace Gita Galermo, Andres Guerrero, Guy Treves, Eshani Nandita, Muchena J. Kailemia, Shawn M Higdon, Tania Pozzo, John M. Labavitch, Alan B. Bennett, and Carlito B. Lebrilla Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.9b00789 • Publication Date (Web): 15 Apr 2019 Downloaded from http://pubs.acs.org on April 15, 2019

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 35 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Analytical Chemistry

Table of Contents 82x41mm (150 x 150 DPI)

ACS Paragon Plus Environment

Analytical Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 2 of 35

A Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria Matthew J. Amicucci†,$, Ace G. Galermo†,$, Andres Guerrero†, Guy Treves†, Eshani Nandita†, Muchena J. Kailemia†, Shawn M. Higdon‡, Tania Pozzo‡, John M. Labavitch‡, Alan B. Bennett‡, Carlito B. Lebrilla*,† AUTHOR AFFILIATIONS †Department

of Chemistry, University of California, Davis, Davis, CA 95616 USA

‡Department

of Plant Sciences, University of California, Davis, Davis, CA 95616 USA

$These

authors contributed equally

*Corresponding

Author: Carlito B. Lebrilla ([email protected])

*To whom correspondence should be addressed. Carlito B. Lebrilla University of California, Davis Department of Chemistry One Shields Avenue Davis, CA, 95616, USA E-mail: [email protected] ABSTRACT


1

Page 3 of 35 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60


The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants. A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment. This synbiotic relationship is facilitated by a polysaccharide, whose complicated structure has been previously unknown. In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation. The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches. This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community. The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.

KEYWORDS: Polysaccharide, monosaccharide, linkage analysis, carbohydrates, mass spectrometry, diazotrophic, nitrogen fixation.


2


Page 4 of 35

INTRODUCTION Polysaccharides are an important class of bioactive compounds that play large roles in hostmicrobe interactions.1-4 However, the elucidation of their structures have remained difficult due to their large size and inherently complicated nature. Defining the structures of polysaccharides has become increasingly important as they dictate how organisms interact with their physical and biological environments. For example, pectin-derived oligogalacturonides have been found to mediate communication between plants and microorganisms that regulate plant growth and development.5-7 Additionally, hemicellulose polysaccharides, such as xyloglucan, bind cellulose fibrils in plant primary cell walls and are responsible for cell growth and expansion.8-10 In the context of mammalian nutrition, the nature of the monosaccharides, linkage positions, and stereochemistry of the glycosidic bonds, dictate digestibility and modulate the gut-microbiome.11-14 This report presents a general liquid chromatography-mass spectrometry (LC-MS)-based workflow for the de novo characterization of structurally diverse polysaccharides. We demonstrate the approach by elucidating the structure of a mucilage polysaccharide secreted from the aerial roots of a landrace maize (Zea mays Y.) endogenous to Sierra Mixe, Mexico. The mucilage is a clear viscous gel found on the aerial roots at each node of the maize stalk and contains a diverse consortia of bacteria whose genomes are enriched in genes responsible for nitrogen fixation.15 This variety of maize is of particular interest for its ability to fix as much as 82% of its nitrogen from the atmosphere.16 Previous efforts in characterizing the mucilage composition revealed that it is rich in galactose, fucose, and arabinose, however, the nature of the glycosidic linkages and monomer arrangements were not elucidated.16 This combination of monosaccharides is not commonly found in plant cell wall polysaccharides and may select for a specific mutualistic bacteria that are uniquely able to degrade and consume the mucilage polysaccharide in exchange for atmospheric nitrogen fixation. Recent work by Pozzo et al. demonstrated that the mucilage and its associated bacteria contain glycosyl hydrolases


3

Page 5 of 35 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60


capable of depolymerizing the same monosaccharides described,17 and further supports the hypothesis that the associated rhizobiome can utilize the mucilage polysaccharide as its sole carbon-source. Thus, elucidation of the mucilage polysaccharide structure has been proposed as the next-step for understanding the mechanism behind the maize host-microbe symbiosis. The development of methods for polysaccharide characterization have lagged behind other macromolecules such as proteins and DNA. Unlike linear and template-derived proteins and DNA, polysaccharides do not consist of a single defined structure, but instead represent a class of macromolecules that share similar but not exact monosaccharide and linkage composition, branching, and degree of polymerization (DP).9,18,19 Due to these additional complications, no single method can characterize an entire polysaccharide structure. Rather, a series of orthogonal tools must be employed to probe specific defining features such as monosaccharide composition, linkage positions, and monomer arrangements. For this reason, it may take several lab groups over several years to elucidate a single polysaccharide structure using outdated analytical methodologies.20-23 Polysaccharide characterization requires analysis of the monosaccharide composition. The current standard method of analysis employs gas chromatography coupled to mass spectrometry (GCMS) or flame ionization detection (GC-FID).20,23-26 However, these methods have difficulty analyzing a broad range of monosaccharides and suffer from tedious derivatization, low sensitivity, and extensive analysis times. Methods involving high-performance anion exchange chromatography paired with pulsed amperometric detection (HPAEC-PAD) avoid chemical derivatization but also suffer in terms of sensitivity and speed.27-30 Furthermore, extensive analysis times of 45-90 minutes are typically required for isomer separation by GC-MS, GC-FID, and HPAEC-PAD, rendering it difficult to obtain adequate experimental replicates. Previous methods for linkage analysis also rely on GC-MS and similarly suffer from low sensitivity and unfavorable chromatographic conditions.21,31 For these reasons, we have recently developed methods for rapid-throughput monosaccharide and linkage analysis that employ 1-


4


Page 6 of 35

phenyl-3-methyl-5-pyrazolone (PMP) derivatization in combination with ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC/QqQ-MS).32,33 A separate but necessary approach for characterizing the monosaccharide arrangements involves depolymerization of the polysaccharide into smaller oligosaccharides by either partial acid or enzymatic hydrolysis followed by analysis of the oligosaccharides.34-40 Unlike the proteolytic enzymes employed in proteomics, a trypsin-equivalent enzyme for polysaccharides does not exist, resulting in the need for multiple enzymes to be employed. Partial acid hydrolysis has been shown to be an attractive method for generating oligosaccharides from polysaccharides.40,41 However, commercial standards are limited and structural databases for the generated oligosaccharides do not exist, resulting in the need for characterization de novo. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques have been highlighted by our lab and elsewhere as an effective platform for de novo oligosaccharide analysis.42,43 In this report, a workflow integrating a series of modern LC-MS/MS techniques was developed to structurally characterize a unique maize mucilage polysaccharide (Figure 1). Partial acid hydrolysis involving trifluoroacetic acid (TFA) was performed to depolymerize polysaccharides into oligosaccharides. Nano-chip high-performance liquid chromatographpaired with quadrupole time-offlight mass spectrometry (nano-HPLC-chip-QTOF MS) was employed to characterize the oligosaccharide structures and construct a library. Semi-preparative anion exchange flash liquid chromatography (AEFLC) paired with monosaccharide analysis was performed to determine if the mucilage is comprised of a single polysaccharide or multiple co-existing polysaccharides. The linkage compositions of maize mucilage grown in both a controlled greenhouse and the native environment were compared. The workflow provides unprecedented characterization of an important root mucilage and provides new insight into the mechanism by which the maize supports and selects for the community of diazotrophic bacteria that it relies on. This workflow is not limited to elucidating mucilage polysaccharides, but will


5

Page 7 of 35 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60


provide faster and more thorough polysaccharide characterizations that will also enhance our ability to monitor diet-microbiome interactions.

Figure 1: Workflow for the structural characterization of the maize polysaccharide using a combination of LC and LC-MS/MS methods. The workflow provides monosaccharide composition, linkage composition, and oligosaccharide structures.

RESULTS The mucilage was obtained from maize aerial roots grown either in Sierra Mixe, Mexico or the University of California-Davis greenhouse. The mucilage production at the two sites were compared with the eventual goal of understanding the role that variations in local conditions and potentially local


6


Page 8 of 35

microbiomes play in mucilage biosynthesis. To better understand those effects, it was first necessary to develop a workflow for elucidating the structures of the mucilage carbohydrates (Figure 1). We used UHPLC/QqQ MS for both monosaccharide and linkage analyses. Nano-HPLC-chip-QTOF MS was used for oligosaccharide arrangement analysis. Anion-exchange flash-LC was used to determine whether the mucilage comprised of a single polysaccharide structure or a mixture of structurally different polysaccharides. Furthermore, unique to this effort was the integration of newly developed LC-MS methods for monosaccharide analysis32, linkage analysis33, and oligosaccharide sequencing that makes the structural elucidation of complicated polysaccharide structures faster and more feasible. The mucilage polysaccharide is comprised of a unique set of monosaccharides The most basic description of a polysaccharide’s structure is the monosaccharide composition. Monosaccharide analysis was performed by using a combination of acid hydrolysis and PMPderivatization followed by UHPLC/QqQ-MS analysis. The method provided broad monosaccharide coverage that included both neutral and acidic monosaccharides and high reproducibility (

Strategy for Structural Elucidation of ... - ACS Publications

Recommend Documents