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LEOXIDAS ZERVAS, I I I L T O N
TvISITZ A X D
Appendix 11
J. P.GREENSTEIN
1'01. s3
Since
Denaturation and Change in Hydrogen-ion Binding.LVhen, in a certain PN-range, the ratio of native to denatured molecules is pH-dependent, denaturation must be accornpanied by a change in the average number of hydrogen ions dissociated from the protein molecules, Using equation Y, A11 of the precedinypaper,4 we hdve
dy/dPH = -2.303 dy/d In H = -2.303 (dyld In Kut,a,i.) ( d In I