J . Am. Chem. SOC.1990, 112, 581 1-5819 Table V. Second-Order Rate Constants k N p (M-' s-I) for Hydrolysis of 4-Nitrophenyl Acetate at 25 O C nucleophile kNP P& (H@) 11
4.1 X
7.3
(NH,)&o"'OH 1.5 x 1 0 - 3 b 6.4 bovine carbonic anhydrase 4.0 X lo2' 1.5 9 9 15.5 OH'At pH 8.2 (50 mM HEPES buffer) and I = 0.10 (NaCIO,). bFrom ref 3.
