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Remediation and Control Technologies
Salinity-aided selection of progressive onset denitrifiers as a means of providing nitrite for anammox Wei Li, Hui Li, Yong-di Liu, Ping Zheng, and James P. Shapleigh Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.8b02314 • Publication Date (Web): 27 Aug 2018 Downloaded from http://pubs.acs.org on August 28, 2018
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Table 1. Summary of nitrogen conversion efficiencies (average ± sd) and sampling information for microbial community analysis during different treatment phases. Phase
HRTa
Salt
-
NO3 -N
-
NO2 -N to -
-
-
-
-
N2 (g) to (NO3 -N
N lost to (NO3 -N
NO2 -N to (NO3 -N
Designations for 16S rDNA analysis (Sample Day)b
(h)
(%)
removal (%)
(Inf. NO3 -N) (%)
conversion) (%)
conversion) (%)
conversion)
Phase 1a (days 1-35)
3.8
0
80.9 ± 10.4
18.6 ± 8.2
50.7 ± 25.3
8.9 ± 14.6
0.22 ± 0.06
1_2 (5th ); 1_3 (35th )
Phase 1b (days 36-67)
1.9
0
83.8 ± 7.3
21.1 ± 9.2
55.6 ± 18.9
7.1 ± 12.9
0.25 ± 0.11
1_4 (60th)
Phase 1c (days 68-101)
1.0
0
85.5 ± 15.6
18.3 ± 5.0
59.0 ± 37.1
10.2 ± 18.8
0.22 ± 0.06
1_5 ( 90th)
Phase 2a (days 102-141)
1.0
1%
84.4 ± 2.5
38.4 ± 10.0
41.4 ± 26.9
5.9 ± 4.2
0.45 ± 0.12
2_1 (105th); 2_2 (130th)
Phase 2b (days 142-175)
1.0
3%
95.6 ± 3.6
60.7 ± 9.5
32.7 ±18.1
6.3 ± 12.7
0.64 ± 0.13
2_3 (160th)
Phase 2c (days 176-205)
1.0
5%
97.4 ± 1.4
72.9 ± 6.6
23.5 ± 16.9
5.5 ± 10.3
0.75 ± 0.07
2_4 (180th); 2_5 (200th)
Phase 3a (days 206-259)
1.0
0%
73.6 ± 14.6
19.3 ± 12.3
49.3 ± 31.0
11.3 ± 11.6
0.23 ± 0.15
3_1 (225th); 3_2 (250th)
Phase 4a (days 260-308)
1.0
5%
94.1± 2.7
63.0 ± 18.6
28.1 ± 24.4
6.9 ± 10.2
0.67 ± 0.21
4_1 (270th); 4_2 (300th)
a Hydraulic b See
retention time Figure 2
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Table 2 Quality and related genome information of MAG bins.
a
Draft genome ID
Taxonomic assignmenta
Genome size (Mbp)
Gene count
Phase 2
Phase 3
Halo1
Halomonas(g)
93.82
1.47
65.4
4.28
3946
36.70%
0.55%
Halo2
Halomonas(g)
97.91
7.63
61.9
5.75
5377
3.82%
0.20%
Azoa1
Azoarcus (g)
93.10
18.57
63.3
4.45
4610
8.0%
17.0%
Completeness Contamination
GC
Abundance (%)
Lowest assigned taxonomic rank – (g) is genus.
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Environmental Science & Technology
Figure 1
Figure 1 Performance of the reactor during different treatment phases (1a, 1b, 1c, 2a, 2b, 2c, 3 and 4). NLR, nitrate loading rate, is defined as the ratio of influent NO3--N to HRT. NRR, nitrate removal rate, is defined as (Inf.NO3- - Eff.NO3-) / HRT. ACR, nitrite accumulate rate, is defined as (Eff. NO2- Inf.NO2-) / HRT. Salinities (w/v) are shown above the corresponding reactor phase.
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Figure 2
Figure 2 Genus-level abundance of 16S rRNA gene-based sequences from the bioreactor biomass. The relative abundance of a genus within a biomass community is defined as the number of sequences affiliated with that genus divided by the total number of sequences in the sample.
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Environmental Science & Technology
Figure 3
Figure 3 Relative abundance comparison of key genes associated with denitrification. Nap, periplasmic nitrate reductase precursor; Nar, cytoplasmic nitrate reductase alpha chain; NirK, copper nitrite reductase; NirS, cytochrome cd1 nitrite reductase; cNor/qNor, nitric oxide reductase cytochrome or quinol-dependent; Nos, nitrous oxide reductase. Student's t test was used to evaluate the difference in gene abundance between phase 3 and phase 2 samples (p
