Technicon Chromatography Corp. - Analytical ... - ACS Publications

May 18, 2012 - Technicon Chromatography Corp. Anal. Chem. , 1960, 32 (7), pp 129A–129A. DOI: 10.1021/ac60163a850. Publication Date: June 1960...
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T E C H N I C O N

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fl UTOMATIC GRflO ϋί;ί V A R I A B L E

D E V I C E · ^ produces any buffer requirement in pH or concentration for chromatography vbhjnghjghjghjghjghjghjghjghjghjgh ghjghjghjghjghghjghjghghghjghjghjghj

DISCRETE BUFFER CHANGEOVERS as an example . . .

to PH to PH

5.28

4.25

from pH 3.25

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A M I N O ACID CHROMATOGRAPHY instead the Autograd automatically sup­ plies a buffer gradient in a continuous, flow.** uninterrupted

Th/s solution » arrangement of pH values in the AUTOGRAD t automatically produces the gradient

below.

The- Autograft* starts the column elufion delivery at pH 3.05, a» shown above, and progressively Un a con­ tinuous flow, increase* to pH 6.85. Not only Is each discrete buffer value systematically produced, but the resulting gradient includes all of the intermediate values as graphed below

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