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The metabolic signature of Primary Biliary Cholangitis and its comparison with Coeliac Disease Alessia Vignoli, Beatrice Orlandini, Leonardo Tenori, Maria Rosa Biagini, Stefano Milani, Daniela Renzi, Claudio Luchinat, and Antonino Salvatore Calabrò J. Proteome Res., Just Accepted Manuscript • DOI: 10.1021/acs.jproteome.8b00849 • Publication Date (Web): 12 Dec 2018 Downloaded from http://pubs.acs.org on December 13, 2018
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Journal of Proteome Research
The metabolic signature of Primary Biliary Cholangitis and its comparison with Coeliac Disease
Alessia Vignoli1,#, Beatrice Orlandini2,#, Leonardo Tenori3,4, Maria Rosa Biagini2, Stefano Milani2, Daniela Renzi2,5, Claudio Luchinat1,4,6,*, Antonino Salvatore Calabrò2,5,*
1Consorzio
Interuniversitario Risonanze Magnetiche di Metallo Proteine (C.I.R.M.M.P.), Sesto
Fiorentino, Italy 2Department
of Experimental and Clinical Biomedical Sciences, University of Florence, Italy
3Department
of Experimental and Clinical Medicine, University of Florence, Florence, Italy
4Magnetic 5Tuscany
Resonance Center (CERM), University of Florence, Sesto Fiorentino, Italy
Referral Center for Adult Coeliac Disease, Florence, Italy
6Department
#AV
of Chemistry, University of Florence, Sesto Fiorentino, Italy
and BO contributed equally to this study.
*AC and CL share the senior authorship of this article.
Correspondence: Antonino Salvatore Calabrò, Department of Experimental and Clinical Biomedical Sciences Viale Pieraccini, 6, 50139, Florence, Italy. E-mail:
[email protected] Telephone: +39 329 256 3790 Claudio Luchinat, Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino (Florence), Italy. Email:
[email protected] Telephone: +39 0554574296.
1 ACS Paragon Plus Environment
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ABSTRACT Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease characterized by ongoing inflammatory destruction of the interlobular bile ducts, eventually leading to chronic cholestasis and biliary cirrhosis. This study primarily aims to define the metabolomic signature of PBC after comparison with healthy controls (HC). Secondly it aims at evaluating the possible metabolic association between PBC and coeliac disease (CD), an immune-mediated disorder frequently associated with PBC. Serum and urine samples from 20 PBC, 21 CD and 19 sex-matched HC subjects were collected. 1H NMR spectra for all samples were acquired, and multivariate statistics was used to evaluate the differences among the three groups and to provide information about the involved metabolites. The classification accuracies to discriminate PBC and HC groups were 78.9-84.6% for serum and 76.9% for urine. In comparison with HC, PBC patient sera were characterized by altered levels (P-value
