THE NITROGEN ISOTOPE (N15) AS A TOOL IN THE STUDY OF THE

Rudolf Schoenheimer, D. Rittenberg, M. Fox, Albert S. Keston, and Sarah Ratner. J. Am. Chem. Soc. , 1937, 59 (9), pp 1768–1768. DOI: 10.1021/ja01288...
2 downloads 0 Views 113KB Size
1i G S

COMMUNICATIONS TO THE EDITOR

These and other results demonstrate the close relationship of nicotinic acid and the amide to black tongue. The fact that nicotinic acid amide is an essential ingredient of the diet is not surprising since it is a component of certain coenzymes. However, the observation that a deficiency of this material may be the cause of black tongue is most interesting. Whether these compounds are equally effective in human pellagra can only be answered by clinical trials.

Val. 59

THE NITROGEN ISOTOPE (NI5) AS A TOOL IN THE STUDY OF THE INTERMEDIARY METABOLISM OF NITROGENOUS COMPOUNDS

Sir: Urey, Fox, Huffman and Thode [THISJOURNAL, 59, 1407 (1937)] have recently succeeded in con-

centrating the nitrogen isotope of atomic weight 15 (N16). Professor Urey has kindly supplied us with a sample of ammonia enriched in N'j. I t had a N14/N16 ratio of 160 as compared to a normal of 266. All isotope analyses were carried DEPARTMENT OF AGRICULTURAL CHEMISTRY OF WISCONSIN C. A. ELVEHJEM out with a mass spectrometer. Glycine and UNIVERSITY R. J MADDEN hippuric acid were synthesized from this ammonia, MADISON, WISCONSIN F. M. STRONG D. W. WOOLLEY For metabolism studies of organic compounds with isotopes it is essential that the labelled atom RNCHIVED Aucusr 16, 1937 be stable and not exchange with the same element of other compounds. We have studied the systems glycine-ammonia, hippuric acid-ammonia, hippuric acid-glycine, and tyrosine-glycine, and A WATER-SOLUBLE LIGNIN SULFONIC ACID have found no exchange of the nitrogen. FROM AN EXTRACTED OAK LIGNIN Two groups of experiments on biological hipSir: puric acid formation were carried out on rats The authors have succeeded in preparing a with the new compounds. The urinary hippuric water-soluble lignin sulfonic acid from an ex- acid, after either feeding or injection of hippuric tracted lignin by the following method: solvent- acid (N'j), contained a mixture of about one-third extracted, oak wood meal was acetylated by a normal acid and two-thirds of the material fed. modification of the method of Suida and Titsch When glycine and benzoic acid were given to[Monatsh., 54, 700 (1929)l and the isolated, gether by the same methods, the urinary hippuric acetylated lignin-carbohydrate complex hydro- acid contained about two-thirds normal hippuric lyzed in cold aqueous acetone solution with so- acid and one-third hippuric acid (N15). The experiments indicate that hippuric acid dium hydroxide. Filtration and acidification of the filtrate gave an oak lignin which could be can be absorbed from the intestinal tract without separated into two fractions: (a) a chloroform- being hydrolyzed, and furthermore suggest that soluble (lo%), and (b) a chloroform-insoluble glycine may be directly utilized for hippuric acid fraction (90%). Anal. (a) OCH3, 23.4%; (b) formation. The nitrogen isotope should prove C, 63.9; H, 6.2; OCHI, 20.6. The fully methyl- to be as useful in the study of the intermediary ated lignins showed C, 65.2; H, 6.8; OCH3, metabolism of nitrogenous compounds as deuter37.6. The oak lignin was readily and completely ium is in the study of fat and sterols. soluble in sodium bisulfite cooking liquor a t 100- DEPARTMENT OF BIOLOGICAL CHEMISTRY COLLEGE OF PHYSICIANS AND SURGEONS 125'. Treatment of the lignin sulfonic acid AND DEPARTMENT OF CHEMISTRY RUDOLF SCHOENHEINER (OCH3, 23.0) (collaboration of W. L. Hawkins) COLUMBIA UNIVERSITY D. RITTENBERG M. Fox with alkali yielded a mixture of equal parts of NEWYORK,N. Y. ALBERT S. KESTON vanillin and syringic aldehyde (total yield 4.6%). SARAH RATNER RECEIVED AUGUST2, 1937 Its properties, as well as those of the lignin from which it is derived, are now being investigated thoroughly on account of the importance of such CATALYTIC ISOMERIZATlON OF n-BUTANE AND data for use in studies on the mechanism of sulISOBUTANE fite pulp manufacture. Sir: Glasebrook, Phillips and Love11 have recently DIVISIONOF INDUSTRIAL AND HAROLD HIBBERT W. H. STEEVESreported [THISJOURNAL, 58, 1941 (1930) J a low CELLULOSE CHEMISTRY MCGILLUNIVERSITY temperature, catalytic isomerization of n-pentane MONTREAL, CANADA t o ioopentane in the presence of aluminum halides. RECEIVED AUGUST (3, 1937