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1:rec f r o m I c v i i l o h c : S i n c e i i i i i l i n w i l l I i y i l r o l y z e t o l e v u l o s e with I i o i l i n g , iitiy r c d i i c t i u t i t e s t s i i i r * o l v i i i g b o i l i r i g o r t l i e irso or s t r o n g rilknlics, ns in l ~ r l r l i r i ps u l i i t i o n , is a p t t o lie rniclcndirip. A l k i i l i e s o r : r i d siilts will hAsteri t l i c h y d r o l y s i s . 'rile iiiiiliii s o l u t i o n iii i n c i l i i i s l r o t i l i l not Ire s t e r i l i z e d i n t h e A r i i i > l d , tliougli n piirrly iiiiiliii soilltino rnity i l c s t e r i l i z e d in i l r r i i t i t o i - l i i w il i i i i i i l e i i p \ v i l l i t l i a i i l l r r l \viitpr. T h i s iiiiiliii sohition twii !licii he i i i l i l c d tn tlic i i ~ c i l i i iwith 11 s!eriliti*il l t i p c t 11s r c q i i i r e i l , T h e IJW. I c r i o l o p i c i i l l r b t or iiiuliii f o r Iiurity is i n o r e s c t i s i t i v c tliiiii iiiiy c l i c r n i c n l tcst. Iiiiiliii c l i i i i i l i l SIIO\V n o iicicl o r ~ i i sp r o i l i i c t i o i i with I ) . ~ a r u / y p h o s a s 13, or I ! . pro1,'u.r. C. S. h~riclpc?h n s routld tliiit i t is n o t so i i i i i c l i n question o r i n t e n s i t y of l i c n t his i t is tlic I c n g t h of t i n i e w l i i c l i t h e ciirlioliydriite i s cuposed t o t h e Iit~iit. A 10 p e r c c i i t s o l u t i o i i of a s i i x i i r eiiii 11c s t e r i l i z e d in t h e i i i i t w l a v e , iI iriiiilc u p w i t h !liufillcd w i t c r . w l i i l c i t iiiriy not IJCpossililc to s t e r i l i z e Q 0 . J per c e n t s o l i r ~ i o r iof t l i c w i n e sugar, i n r n e d i i i , i n tlic hriiolil. T h i s \V~IEII5 of irciils iiiii! illso s i i l t s i n t h e rireriin. is I r r o l i i i l i l y IIIICt o s r i i i i l l tr v v c r tlic*re is (Iiitigcr 01 l i y i l r o l y s i s , t l i c n i r d i u iind it I O I i c r c e n t s o l u t i o n of t h e c a r l i o l i y d r o t c sliotiltl b e s t c r i l i z c d s e p n r o t c l y . 'Plic w R n r c a n then be added t o c r i c l i t t i l i c w i t h a stcrilizcrl p i j i c t , 0 , 5 p e r c e n t or 1 p e r c e n t as r c q u i r e d . T l i c n1ior.c tecliniquc should l i e i i s c d i n t e s t i n g t h e inulin. Llal:c ti11 n I O p e r c u r i t s o l u t i o i i or t h e i n t r l i n b y p o i r r i t i K 50 cc. or boilinp d i ~ t i l l e c ln n i c r on .?I E. of i i i i i l i r i . S t e r i l i z e n t l0O0 C. f o r 15 min. Siin:ir.rrcc I i r o t l i ( f r c c f r o m tiiusclc sugnr Iiy f c r m e n t a t i o n w i t h B. coli) i i i i i s t b e t i s c d , t i t r a t e d t o p I f i . ( i . Aik! 0.8 cc. or ii 0.02 p e r c e n t s o l u t i o n n l ~ i i0i . 5 cc. of a 0.02 p c r cent o r jihciiul r e d ( ~ i l i c t i o l ~ t i ! ~ a i i c ~ i l i t l iuiid soliitioir 01 h r o i n o c r c s o l p u q r l c (i!ibroiiin-o-crc?iolsiilfoiicplitliiileiii~.Pour i i i t o f c r r i r i ~ r i t r i l i o r t~l i h c s nnd s i r r i l i z c . T h r e e cc. o f the s t e r i l i z e d iniilin s o l u t i o n n r c iiilrlerl t o t h c I i r o t l i i n ii sterile inuiiiier. hlix nncl i n o r t i l u t e w i t h U . purii!yphoid /I. ICrutiiiiie t u l i c s nfier 2 1 Iirs.. .IS h r s . . u n d o n e week for (1) A r i d i t y tis i i i d i c n t c i l l i y cliiinfie i n c o l o r or i n i l i c i i t o r . (II-ion conc c i i t r i i t i o u inny lie d e t e r i i i i i i c i l Iiy t h e C l u r k niid 1.tibr. i n e t l l o c l . ) ( 2 ) rh.; I ) r o d i i c t i n n . U r i i i i o c i i l n t c i l c o n t r o l s n r c '0 be kclit a t i n c u b n t o r l e ni ricrii tu re ut s i i n i c t i m e . 8 I n u l i n rrcc frotti r c i h i c i r i g sugiirs shoiild riot show ncitl I i r o t i t t c t i o n nnd nf course iio pns. l'hc r o t a t i o n of i i n i l i r i lins n o t I ~ c e r ni c c u r u t c l y dctcrriiiried. in riirigc I O o . ' A 10 i l i f f i w t i t i i i v c s t i g i i t o r s r c p o r t i i i x r o t i ~ t i o t i viiryiiix ~ iicr c e n t s o l u t i o n of high grurtc iniiliii tnode frorri clnhlins s l i o ~ l dhove B specific r o t r i t i o i t of n o t I c s tlinii -:KI~. l'lie p r o d u c t i o n of r t i l i i c i n p siiKnrq i n inillin lip h o i l i n ~s c c r i ~t ~ o depend irlmii t l i c c o n d i t i o n of tlic urixiiiiil s u l i s t u r i c e from w h i c h t h e iiiuliii is t a k c n . 'The itriiliri i n rlnhliii.r ilri): i i i t l i c fall liepins i i n i i i e r f i i i t c l y to c h a i i g c o v c r i n t o co~t~pouri~ aiialogoiis ls io t h e lony w r i e s of d e x t r i n s i n t c r n r e i l i n t c I x t w e c o b t i i r c h o i i i l d i i c o r e . On Iroiliiig. t l i c s c cooipoiiiidc, o r iit lciist sotnc of t l i c n i . r s p e v i i i l l y i n t l i c prescncc of s a l t s f r o i i i tire j i i i c c nncl traces or o r g n n i c ncidu Ioiiiid i n t h c jiiicc, will I i y d r u l y r c t o Icvuluse. 'I'herclore. b o i l i n g i n i i l i n , espcciully w i t l i 1:ciilitifi solittioil, ciiii give r c i l i i c i n p su,qnrs. The only r c liulile inctliorl fur testing t h e reiliicinx suxur in iiniliri therefore is bactcrio. logicill.
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