Subscriber access provided by Eastern Michigan University | Bruce T. Halle Library
Article
Trans-10, cis-12 Conjugated Linoleic Acid Affects Expression of Lipogenic Genes in Mammary Gland of Lactating Dairy Goats Huaiping Shi, tianying zhang, cong li, jianjue wang, jiangtao huang, and zhongyang Li J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b02377 • Publication Date (Web): 11 Oct 2017 Downloaded from http://pubs.acs.org on October 17, 2017
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 30
Journal of Agricultural and Food Chemistry
1
Trans-10, cis-12 Conjugated Linoleic Acid Affects Expression of Lipogenic Genes
2
in Mammary Gland of Lactating Dairy Goats
3 4
Huaiping Shi, Tianying Zhang, Cong Li, jianjue wang, jiangtao huang, zhongyang Li
5 6
Running title: Conjugated Linoleic Acid blocks milk fat metabolism
7 8
College of Animal Science and Technology, Northwest A&F University, Yangling,
9
Shaanxi 712100, China
10 11
Corresponding author. Tel.:+86-29-8709-2102; fax: +86-29-8709-2164
12
Email:
[email protected];
13
[email protected];
14
[email protected] ;
15
[email protected];
16
[email protected];
17
[email protected] 18 19 20 21 22 1
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
23
ABSTRACT
24
The molecular mechanisms on milk fat depression (MFD) in response to trans-10,
25
cis-12 conjugated linoleic acid (t10c12-CLA) supplementation in ruminants, were
26
elucidated in this research with dairy goats. Thirty two-year-old Xinong Saanen dairy
27
goats (40 ± 5 days in milk (DIM)) at peak lactation stage were assigned to a 3×3 Latin
28
Square design (14-d treatment period followed with 14-d washout). Three CLA
29
treatments included a) Control, fed the basal diet only without CLA supplementation;
30
b) orally supplemented with 8 g/d of lipid-encapsulated CLA (low dose; CLA-1); and
31
c) 16 g/d of lipid-encapsulated CLA (high dose; CLA-2). Expression levels of fatty
32
acid metabolism genes in the mammary tissues were analyzed by RT-qPCR in 3 goats
33
on d 1 and the other 3 goats on d 14 in each group after the discontinuation of CLA
34
treatment in the third experimental period. Dietary supplementation of CLA led to a
35
significant decrease of milk fat compared with the control (P ＜ 0.05). Milk fat
36
concentrations in CLA-1 and CLA-2 groups were 2.74% and 2.42% respectively,
37
while 2.99% in control group. Decreases in short- and medium-chain fatty acids (
