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Transcriptome analysis of hepatopancreas from the Cr (VI)stimulated mantis shrimp (Oratosquilla oratoria) by Illumina PairedEnd Sequencing: Assembly, Annotation and Expression Analysis Daizhen Zhang, Jun Liu, Tingting Qi, Baoming Ge, Zhengfei Wang, Senhao Jiang, Qiuning Liu, Huabin Zhang, Ding Ge, and Boping Tang J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b05074 • Publication Date (Web): 09 Feb 2018 Downloaded from http://pubs.acs.org on February 11, 2018
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Transcriptome analysis of hepatopancreas from the Cr (VI)-stimulated mantis
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shrimp (Oratosquilla oratoria) by Illumina Paired-End Sequencing: Assembly,
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Annotation and Expression Analysis
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Daizhen Zhang1†, Jun Liu2†, Tingting Qi1, Baoming Ge1, Zhengfei Wang1, Senhao Jiang1, Qiuning
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Liu1, Huabin Zhang1, Ge Ding3*, Boping Tang1*
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1.Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Provincial Key Laboratory of
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Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center
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for Coastal Bio-agriculture, Yancheng Teachers University, Yancheng 224051, China; 2. Key
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Laboratory of Biotechnology in Lianyungang Normal College, Lianyungang 222006, China; 3.
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Chemical and Biological Engineering College, Yancheng Institute of Technology, Yancheng
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224003, China
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†
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* Correspondence author:
[email protected] These authors contributed equally to this work.
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract: Cr (VI), the pathogenicity factor, which is widely known to cause toxic
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effects in living organisms. Given the economic importance of the mantis shrimp
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(Oratosquilla oratoria), the understanding of impacts by Cr (VI) is considered
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important. In this study, transcriptome of mantis shrimp was characterized by a
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comparison between control and Cr (VI)-treated samples using RNA-seq approach.
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Totally, 88,234,826 bp and 13.24G clean reads were obtained. The total length and
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number of unigenes were 68,411,206 bp and 100,918, respectively. The maximal and
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average length of unigenes was 24,906 bp and 678 bp, respectively (N50, 798 bp).
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7115 of these unigenes accounted for 7.05% of the total were annotated in all
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databases. After annotation of assembled unigenes, 35,619 of them were assigned into
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3 functional categories and 56 subcategories using Gene Ontology; 18,580 of them
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were assigned into 26 functional categories using Clusters of Orthologous Groups of
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proteins; 16,864 of them were assigned into 5 major categories and 32 subclasses
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using KEGG. Finally, 1,730 genes were differentially expressed (DGEs) and 9
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up-regulated
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Ligand-receptor Interaction, Pancreatic Secretion, Tyrosine Metabolism, Amoebiasis,
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ECM-receptor Interaction, Riboflavin Metabolism, Amino Sugar and Nucleotide
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Sugar Metabolism and AGE-RAGE Signaling Pathway in Diabetic Complications)
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were significantly enriched (q
