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Transepithelial Transport of YWDHNNPQIR and its Metabolic Fate#with Cytoprotection against Oxidative Stress#in Human Intestinal Caco-2 Cells Feiran Xu, Lifeng Wang, Xingrong Ju, Jing Zhang, Shi Yin, Jiayi Shi, Rong He, and Qiang Yuan J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b04731 • Publication Date (Web): 20 Feb 2017 Downloaded from http://pubs.acs.org on February 22, 2017
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Transepithelial Transport of YWDHNNPQIR and its
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Metabolic Fate, with Cytoprotection against Oxidative
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Stress, in Human Intestinal Caco-2 Cells
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Feiran Xu, Lifeng Wang*, Xingrong Ju, Jing Zhang, Shi Yin, Jiayi Shi, Rong He,
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Qiang Yuan
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(College of Food Science and Engineering/Collaborative Innovation Center for
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Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality
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Control and Processing, Nanjing University of Finance and Economics, Nanjing
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210023, People’s Republic of China)
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Title running header:
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Absorption and Antioxidation of YWDHNNPQIR
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT
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Studies on the antioxidant peptides extracted from foodstuff sources have included
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not only experiments to elucidate the chemical characteristics of them but have also
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investigated their bioavailability and intracellular mechanisms. This study was
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designed to clarify the absorption and antioxidative activity of YWDHNNPQIR
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(named RAP) which derived from rapeseed protein using Caco-2 cells transwell
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model. Results showed that 0.8% RAP (C0=0.2mM, t=90min) could maintain the
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original structure to across the Caco-2 cell monolayers via the intracellular
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transcytosis pathway, and the Papp (drug apparent absorption rate) was (6.6 ± 1.24)
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×10-7 cm/s. Three main fragments (WDHNNPQIR, DHNNPQIR, and YWDHNNPQ)
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and five modified peptides derived from RAP were found in both the apical and
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basolateral side of the Caco-2 cells transwell model. Among these new metabolites,
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WDHNNPQIR had the greatest anti-oxidative activity in Caco-2 cells apart from the
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DPPH assay. With a RAP concentration of 200 µM, there were significant differences
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in four antioxidative indicators (T-AOC, GSH-Px, SOD, and MDA) compared to the
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oxidative stress control (P
