Use of mtDNA Direct Polymerase Chain Reaction (PCR) Sequencing

1662

J. Agric. Food Chem. 1998, 46, 1662−1669

Use of mtDNA Direct Polymerase Chain Reaction (PCR) Sequencing and PCR-Restriction Fragment Length Polymorphism Methodologies in Species Identification of Canned Tuna J. Quinteiro,† C. G. Sotelo,*,‡ H. Rehbein,§ S. E. Pryde,| I. Medina,‡ R. I. Pe´rez-Martı´n,‡ M. Rey-Me´ndez,† and I. M. Mackie| Departamento de Bioquı´mica y Biologı´a Molecular, Facultad de Biologı´a, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain; Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain; Bundesforschungsanstalt fu¨r Fischerei, Institut fu¨r Biochemie und Technologie, Palmaille 9, 22769 Hamburg, Germany; and Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB2I 9SB, Scotland, U.K.

Identification of six canned tuna species using DNA-based methodology was studied. DNA was degraded during the canning process of fish muscle: DNA fragment sizes that ranged from