Preface
Downloaded by 80.82.77.83 on May 28, 2018 | https://pubs.acs.org Publication Date: January 24, 1990 | doi: 10.1021/bk-1990-0419.pr001
THE
RECENT
ADVANCES
IN
GENETIC
ENGINEERING
AND
CELL
CULTURE
that have spawned the new biotechnology industry have also stimulated new thinking and research in downstream processing. This new research and development, which focuses on separation and purification of biological materials, is welcome and much needed, in view of the central role of bioseparation engineering in the process economics of biotechnology. Downstream processing is commonly classified into four distinct steps: broth conditioning and removal of insolubles; isolation of the desired product (including clarification and extraction); purification with high-resolution techniques; and polishing. Of these steps, isolation and purification currently enjoy the most attention from researchers. The authors of this book have made further progress in their respective research programs since the symposium on which this book is based. These revisions and new data are included in this book. Most chapters include data that have not been published before. Moreover, each chapter has received two reviews by relevant experts. The aim of this book is not to provide an exhaustive treatise on all areas of isolation and purification of biotechnology products, but to present the spectrum of current thinking and activities on bioseparations, specifically of large molecules such as proteins and polysaccharides. The chapters are divided into three categories: extraction and membrane processes, processes using biospecific interaction with proteins, and novel isolation and purification processes. A n overview chapter by Hamel and Hunter presents the state of the art of research on bioseparations. Extraction processes using biphasic aqueous systems, liquid membranes, reversed-micellar systems, and membrane processes are all being actively studied. Significant advances in these topics, including predictive mathematical models, are presented in the first section. The second section includes several papers on affinity and other interaction techniques that are finding uses in protein purification. In the last section, we offer several reports that delineate advances in isolation and purification processes such as electrophoresis and chromatography. vii
Hamel et al.; Downstream Processing and Bioseparation ACS Symposium Series; American Chemical Society: Washington, DC, 1990.
We gratefully acknowledge the assistance of our reviewers, whose insight and guidance have enlightened the editors and authors alike. We thank the authors for their special assistance generously extended. Finally, we are indebted to Cheryl Shanks of the A C S Books Department for her patience and many helpful hints during the preparation of this book.
JEAN-FRANÇOIS P. HAMEL Massachusetts Institute of Technology Cambridge, M A 02139
Downloaded by 80.82.77.83 on May 28, 2018 | https://pubs.acs.org Publication Date: January 24, 1990 | doi: 10.1021/bk-1990-0419.pr001
JEAN B. HUNTER Cornell University Ithaca, N Y 14853
SUBHAS K. SIKDAR National Institute of Standards and Technology Boulder, C O 80303 November 6, 1989
viii
Hamel et al.; Downstream Processing and Bioseparation ACS Symposium Series; American Chemical Society: Washington, DC, 1990.
