Effect of magnesium on the properties of zinc alkaline phosphatase

structural stability, or both. The importance of zinc both in catalysis and structure sta- bilization of E. coli alkaline phosphatase has been recogni...
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Effect of Magnesium on the Properties of Zinc Alkaline Phosphatase' William F. Bosron,: Richard A. Anderson,$ Michael C. Falk,:] F. Scott Kennedy,i and Bert L. Vallee*

ABSTRACT: Alkaline phosphatase of Escherichia coli, isolated by procedures which do not alter its intrinsic metal content, contains 4.0 It 0.3 g-atoms of tightly bound zinc per mole (Kd