Effect of Receptor Occupancy on Folate Receptor ... - ACS Publications

Jan 21, 2014 - ABSTRACT: The folate receptor (FR) is a GPI anchored cell surface glycoprotein that functions to facilitate folic acid uptake and media...
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Effect of Receptor Occupancy on Folate Receptor Internalization N. Achini Bandara, Michael J. Hansen, and Philip S. Low* Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States ABSTRACT: The folate receptor (FR) is a GPI anchored cell surface glycoprotein that functions to facilitate folic acid uptake and mediate signal transduction. With the introduction of multiple folate-targeted drugs into the clinic, the question has arisen regarding how frequently a patient can be dosed with a FR-targeted drug or antibody and whether dosing frequency exerts any impact on the availability of FR for subsequent rounds of FR-mediated drug uptake. Although the rate of FR recycling has been examined in murine tumor models, little or no information exists on the impact of FR occupancy on its rate of endocytosis. The present study quantitates the number of cell surface FR-α and FR-β following exposure to saturating concentrations of a variety of folate-linked molecules and anti-FR antibodies, including the unmodified vitamin, folate-linked drug mimetics, multifolate derivatized nanoparticles, and monoclonal antibodies to FR. We report here that FR occupancy has no impact on the rate of FR internalization. We also demonstrate that multivalent conjugates that bind and crosslink FRs at the cell surface internalize at the same rate as monovalent folate conjugates that have no impact on FR clustering, even though the multivalent conjugates traffic through a different endocytic pathway. KEYWORDS: receptor recycling, folate receptor endocytosis, ligand targeted drugs, antibodies to folate receptors, ligand valency on nanomedicines



used to visualize sites of inflammation25 and localize malignant disease,23,26,27 whereas folate-conjugated therapeutic agents have been exploited to destroy FR-α expressing tumor cells28,29 and inactivate FR-β expressing inflammatory macrophages.14,16 A variety of folate-targeted molecules are currently undergoing human clinical trials. Because the rate of FR internalization and trafficking can influence the frequency of folate conjugate dosing (i.e., there is no merit in injecting a patient with a folate−drug conjugate more frequently than empty FR return to the pathologic cell surface following endocytosis), several studies have examined the rate and routes of FR trafficking in physiologically relevant systems.24,30−32 Results from these studies demonstrate that FR traffic through different intracellular compartments depending on the number of folates tethered to the targeted conjugate, with monovalent folate−drug conjugates trafficking through a recycling center before returning to the cell surface,24,31,33 and multivalent FR ligands trafficking through multivesicular bodies prior to deposition in lysosomes.34−37 Importantly, despite the detailed nature of the above studies, the effect of folate conjugate size and valency on its rate of internalization and recycling has never been examined. In this paper, we explore the kinetics of internalization of both FR-α and FR-β following their ligation to a variety of folate-linked molecules and antifolate receptor antibodies, including the free unligated

INTRODUCTION The folate receptor (FR) constitutes a family of four homologous proteins that are thought to bind folic acid and its physiologic congeners.1,2 FR-α is found on the apical surfaces of certain epithelial cells, where it is largely inaccessible to folates in the bloodstream.3,4 It is also overexpressed on a variety of epithelial-derived cancers, where it can be readily targeted by intravenously injected folate-linked drugs.5−7 FR-β is primarily expressed on activated macrophages8 which populate almost all autoimmune and inflamed tissues and mediate many of the destructive processes responsible for disease symptoms.9−11 Examples of inflammatory diseases caused or worsened by FR+ activated macrophages include rheumatoid arthritis, ulcerative colitis, atherosclerosis, multiple sclerosis, and psoriasis among others.12−18 FR-γ has been detected in the bone marrow from whence it may be released into circulation,19 but whether it facilitates folate uptake is not known. FR-δ has been found primarily on regulatory T cells20 and like FR-γ has no known function. Only FR-α has been shown to be involved in signal transduction,21 but the possible participation of FR-β, FR-γ, or FR-δ in transmembrane signaling has never been examined. Because of the limited expression and/or accessibility of FR in healthy human tissues, both FR-α and FR-β have been exploited for targeted drug delivery to cancer tissues and sites of inflammation, respectively.22 For this purpose, folate is linked to a therapeutic or imaging agent and injected into the diseased host, where it is either captured by FR on the pathologic cell surface or rapidly excreted from the body. Radioactive13,15 and fluorescent23,24 folate conjugates have been © 2014 American Chemical Society

Received: Revised: Accepted: Published: 1007

November 4, 2013 January 16, 2014 January 21, 2014 January 21, 2014 dx.doi.org/10.1021/mp400659t | Mol. Pharmaceutics 2014, 11, 1007−1013

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targeting ligands per liposome for a folate-targeted lipid concentration of ∼1.5 μM in the stock suspension. Evaluation of the Effect of Receptor Occupancy on Receptor Internalization. Each cell type to be investigated was plated in a six-well plate at a density of 50 000 cells/well and allowed to adhere overnight. Individual wells were either left untreated (controls) or incubated in 100 nM folic acid or folate rhodamine for 20 min, 4 h, or 24 h at 37 °C. For analyses of liposome uptake, 100 μL of 2 mg/mL folate-conjugated liposome stock suspension was added to each well prior to execution of the same incubation procedure. Following incubation, wells were washed thoroughly with PBS to remove unbound ligand, after which cells were removed from the plate by scraping, centrifuged to form a pellet, and resuspended in cold (4 °C) folate-deficient culture medium to block further FR trafficking. Folate receptors accessible on the cell surface were then labeled with either mAb343 followed by fluoresceinlabeled goat antimouse secondary antibody (KB and IGROV cells) or m909-FITC (CHO-β cells) by further incubation at 4 °C for 1 h. After washing with PBS to remove unbound antibody, fluorescently labeled FR in all cell samples were quantitated on a Becton Dickinson FACS Caliber flow cytometer. Ten thousand cells were counted from each sample, and three samples from each treatment condition were evaluated. CellQuest software was used for data collection, and FlowJo software was employed for data analysis. Graphing and statistical calculations of the analyzed data were performed using GraphPad Prism software.

vitamin, folate-linked small molecules, multifolate derivatized nanoparticles, and monoclonal antibodies to FR.



EXPERIMENTAL PROCEDURES Cell Lines and Culture. All FR positive cell lines were maintained in the Cell Culture Facility of the Purdue University Department of Chemistry. KB and IGROV cells were maintained in folate-deficient RPMI 1640 medium (Invitrogen, Grand Island, NY) supplemented with 10% heat inactivated fetal bovine serum (Sigma Aldrich, St. Louis, MO), penicillin (50 units/mL), and streptomycin (50 μg/mL). Chinese hamster ovary (CHO) cells stably transfected with human FR-β (generous gift from Manohar Ratnam, Karmanos Cancer Center, Detroit, MI) were maintained in folate-deficient RPMI 1640 medium (Invitrogen, Grand Island, NY) supplemented with 10% heat inactivated fetal bovine serum (Sigma Aldrich, St. Louis, MO), 0.15 mg/mL L-proline, 10 nmol/L N5-formyl tetrahydrofolate, 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma Aldrich, St. Louis, MO). All cell lines were passaged continuously in a monolayer and cultured at 37 °C in a humidified atmosphere containing 5% CO2. Antibodies and Reagents. Folic acid was purchased from Sigma Aldrich (St. Louis, MO) and dissolved in pH adjusted deionized water. The hybridoma cell line that produces a mouse monoclonal antibody to human FR-α (mAb 343) was a generous gift from Wilbur Franklin (University of Colorado), and folate-fluorescein (EC17) was kindly provided by Endocyte, Inc. (West Lafayette, IN). Fluorescein-labeled goat antimouse IgG antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Human antihuman mAb against FR-β (m909) was developed in collaboration with Dr. Dimiter Dimitrov (National Institutes of Health, Frederick, MD) and labeled with fluorescein isothiocyanate.9 Folate Conjugates. A water-soluble monovalent folaterhodamine conjugate with nanomolar FR affinity (Figure 1A)



RESULTS Effect of Folic Acid on the Kinetics of FR-α Internalization. For many cell surface receptors, the rate of receptor internalization is strongly dependent on both receptor number and receptor occupancy.38,39 Although previous studies have demonstrated that the rate of FR internalization is independent of receptor number,30 no information is currently available on the impact of receptor occupancy on the rate of FR endocytosis. To obtain this information, KB and IGROV cells (which express high and low levels of FR-α, respectively) were incubated in vitro with a saturating concentration (100 nM) of free folic acid for 20 min, 4 h, or 24 h at 37 °C. Cell surface FR were then quantitated by flow cytometry using a noncompeting anti-FR-α primary antibody (mAb343) followed by labeling with a fluorescein-conjugated goat antimouse secondary antibody. If folic acid binding were to induce FR internalization, a decrease in available FR on the cell surface would be expected as exposure to folate/folate conjugate proceeded. Moreover, if resting state FR levels were to impact the rate of ligand-induced receptor endocytosis, differences between FR internalization by KB and IGROV cells would be anticipated. As seen in Figure 2, the rate of FR-α internalization is not altered by folic acid binding, since cell surface FR-α numbers remain similar to their levels in untreated cells at all of the time points tested. Furthermore, the level of FR expression in untreated cells must exert little influence over the kinetics of receptor internalization, since cell lines that express high and low levels of FR display the same insensitivity to receptor saturation. Effect of Folic Acid on the Kinetics of FR-β Internalization. Although FR-β exhibits similar nanomolar affinity for folic acid to FR-α,40 its greatly reduced level of expression,41 its unique manifestation on activated immune cells,8−11 and its rapid rate of internalization12,30 raise the question of whether the response of FR-β to saturation with folic acid might differ

Figure 1. General structures of (A) folate−rhodamine conjugate and (B) folate−PEG liposomes.

was synthesized as previously described.24,26 Folate-targeted polyethylene glycol-derivatized liposomes were also prepared according to previous procedures,35 with ∼3.5% of the phospholipids derivatized with PEG and ∼10% of the PEGylated lipids further conjugated to folic acid. Assuming ∼80 000 lipids per liposome, this calculates to ∼280 folate 1008

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Figure 2. Quantitation of cell surface FR-α after incubating KB cells (A) and IGROV cells (B) with a saturating concentration (100 nM) of folic acid for 20 min, 4 h, and 24 h. Cell surface FR were quantitated by flow cytometry using a noncompeting monoclonal anti-FR-α antibody, as described in the Methods. Mean fluorescent intensities from three samples were averaged and graphed as a percentage of the average fluorescence intensity measured at the 0 min time point, that is, before addition of free folic acid.

Effect of Folate Conjugate Valency on the Kinetics of Receptor Endocytosis. Cross-linking or clustering of cell surface receptors using multivalent ligands has been known for years to accelerate receptor endocytosis and trafficking to lysosomes.34,36,42 For example, multivalent lectins and antibodies that can bind multiple receptors simultaneously have been observed to induce localized receptor “patching” followed by a more global receptor “capping” prior to receptor internalization and degradation.43 Because many laboratories have exploited folate to target nanoparticles to pathologic cells, invariably derivatizing their nanomedicines with multiple folates to increase binding avidity, the concern has naturally arisen whether such multivalent formulations might induce accelerated depletion of FR from the cell surface, preventing or at least delaying the ability to target additional nanomedicines to the same FR-expressing cells. To address this concern, we have compared the number of cell surface FR following incubation of cells with a monovalent ligand (folate−rhodamine) to their number following incubation with a multivalent ligand (folatetargeted liposomes). As seen in Figure 4, only minor differences are seen in the number of residual cell surface FR following incubation with either monovalent (panel A) or multivalent (panel B) folate conjugates, suggesting that cell surface receptor depletion commonly observed with other multivalent ligands does not occur when FR is the targeted receptor. In the case of monovalent folate−rhodamine, the detected variation in cell surface FR-α (KB) and FR-β (CHO-β) levels following different incubation times are minor and probably a consequence of experimental variability. Even in the case of the multivalent liposomes, the decrease in cell surface FR at early time points is reversed at 24 h, suggesting any real effect may be transient at best. Moreover, although receptor numbers differ significantly with IGROV cells and the receptor sequence differs in CHO-β cells, similar insensitivities to folate conjugate valency are observed. We, therefore, conclude that conjugate valency does not have a significant effect on the rate of FR internalization. Impact of Antibody Binding on FR Endocytosis. Because several anti-FR antibodies have recently entered clinical trials,44 and since antibodies commonly induce the endocytosis of their targeted receptors,45 we felt it might be

from that of FR-α. To evaluate the impact of FR-β occupancy on its rate of internalization, a CHO-K1 strain that was stably transfected with human FR-β was incubated with a saturating concentration of folic acid and examined for cell surface FR-β using a noncompeting monoclonal antibody to FR-β (m909).9 As seen in Figure 3, the level of FR-β on CHO-β cell surfaces is

Figure 3. Quantitation of cell surface FR-β after incubating CHO-β cells with saturating folic acid for 20 min, 4 h, and 24 h, as determined by flow cytometry following labeling of extracellular receptors with m909-FITC. Mean fluorescent intensities from three different samples were averaged and graphed as a percentage of the average fluorescence intensity measured at the 0 min time point, that is, before addition of free folic acid.

independent of the time and extent of FR-β saturation with folic acid. Thus, m909-FITC binding remains essentially the same in the absence of added folic acid (0 min time point) as seen following 24 h exposure to saturating levels of folic acid (24 h time point). Moreover, there is no significant difference in cell surface FR-β between cells incubated for 20 min in saturating folic acid and those incubated for 4 or 24 h with the vitamin. Based on these observations, we suggest that the rate of internalization of FR-β, like that of FR-α, is not altered by changes in the level folic acid binding, but instead recycles at a steady rate, regardless of receptor occupancy. 1009

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Figure 4. Quantitation of cell surface FR-α and FR-β following incubation of cells with folate−rhodamine (A) or folate-labeled liposomes (B) for the indicated times. As in previous figures, cell surface FR were labeled with mAb343 or m909-FITC and quantitated by flow cytometry. Mean fluorescent intensities from three samples were averaged and graphed as a percentage of the average fluorescence intensity measured at the 0 min time point, that is, before the addition of free folic acid.

prudent to include in our study an examination of the impact of antibody binding on FR endocytosis. For this purpose, KB and CHO-β cells were incubated with saturating concentrations of mAb343 and m909, respectively, and subsequently labeled with folate−fluorescein (EC17) to quantitate the number of cell surface FR. As seen in Figure 5, no differences in cell surface FR were observed following incubation with either antibody at any

of the three incubation times, demonstrating that antibody binding also has no impact on FR internalization.



DISCUSSION

The rate of internalization and trafficking of most cell surface receptors may depend on multiple parameters, including: (i) the nature of the ligand,46 (ii) the level of receptor occupancy,38,47 and (iii) the degree of receptor clustering 1010

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Figure 5. Evaluation of cell surface FR after incubation of KB (A, C) and CHO-β (B, D) cells with mAb343 or m909, respectively, for 20 min, 4 h, or 24 h. Cell surface FR was quantitated by incubation of the cells with saturating folate−fluorescein followed by measurement of FITC fluorescence by flow cytometry. Histograms of representative samples from each incubation time (open histograms) are overlaid with the histograms of unlabeled control cells (filled histograms). The 0 min incubation plot shows representative histograms from cells not incubated with antibody. C (KB) and D (CHO-β) are graphical representations of the flow cytometry histograms A and B, respectively.

induced by its binding ligand.48 Thus, natural agonists commonly promote rapid receptor endocytosis, while antagonists generally do not.49,50 High levels of receptor occupancy may also induce internalization and destruction of some occupied receptors, while low levels of receptor saturation may stimulate greater receptor trafficking to recycling endosomes and subsequent return of the receptor to the cell surface.51 Finally, the degree of ligand-induced receptor clustering can also influence receptor fate, with some clustered receptors further aggregating into cell surface patches prior to endocytosis43 and others either internalizing immediately or not endocytosing at all. Not surprisingly, with the many possible effects that different ligands can exert on the endocytosis and trafficking of their cognate receptors, we wondered whether the amount or nature of an injected folatetargeted drug would impact the availability of FR on the targeted cell surface. Thus, if folate−drug conjugate binding were to lead to significant receptor down-regulation, the interval between folate conjugate dosing might have to be lengthened to allow for cell surface FR levels to recover. The unanticipated result from this study was that neither FR occupancy nor folate conjugate valency has any significant effect on FR levels at the cell surface. These data suggest that FR endocytosis occurs at a constitutive rate, regardless of FR occupancy or cross-linking due to multivalent ligand binding. They also suggest that if FR cross-linking induces FR trafficking to lysosomes, as suggested by studies with multivalent folatetargeted nanoparticles,35,36 the synthesis of new FR must compensate for any FR that are degraded. As a consequence,

selection of an optimal frequency for folate conjugate dosing need not involve consideration of folate conjugate concentration or valency. Rather, the only variable that must apparently be evaluated when dosing frequency is optimized constitutes the rate at which the FR naturally recycles in each particular tumor type. Based on previous studies of several human tumor xenografts in live mice, this recycling rate may vary from 8 to 12 h.30



AUTHOR INFORMATION

Corresponding Author

*Purdue University, Department of Chemistry, 560 Oval Drive, West Lafayette, IN 47907-2084. Phone: 765-494-5273. Fax: 765-494-5272. E-mail: [email protected]. Present Address

M.J.H.: BASF, 26701 Telegraph Road, Southfield, MI 48034− 2442. Notes

The authors declare the following competing financial interest(s): The studies reported in this publication were supported by a grant from Endocyte, Inc. Endocyte, Inc. is a biopharmaceutical company that develops folate targeted therapies for the treatment of cancer.



ACKNOWLEDGMENTS We thank Endocyte Inc. (West Lafayette, IN) for their generous financial support and the gift of folate-FITC (EC17). We are grateful to Dr. Dimitri Dimitrov for providing the 1011

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m909-FITC antibody. We are also grateful to Drs. Venkatesh Chelvam and Scott Poh for synthesizing the folate-rhodamine conjugate and the folate-targeted liposomes used in the experiments. Furthermore, we thank Robert Reason for his indispensable help with creating the table of contents figure.



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