5056
Biochemistry 1994, 33, 5056-5062
Effects of a Nonionic Detergent on Calcium Uptake by Cardiac Microsomes? Yong-Zhi Lu and Madeleine A. Kirchberger' Department of Physiology and Biophysics, The Mount Sinai School of Medicine of the City University of New York, One Gustave L. Levy Place, New York, New York 10029 Received October 14, 1993; Revised Manuscript Received February 1 1 , 1994"
ABSTRACT: W e investigated the effects of the nonionic detergent octaethylene glycol monododecyl ether
(C&) on the sarcoplasmic reticulum calcium pump in cardiac microsomes in view of its specific effects on different ATP-accelerated steps in the catalytic cycle of the Ca-ATPase in leaky fast skeletal muscle microsomes. At low concentrations of MgATP2- (
