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Food and Beverage Chemistry/Biochemistry
Effects of Protein-Derived Amino Acid Modification Products Present in Infant Formula on Metabolic Function, Oxidative Stress and Intestinal Permeability Using Cell Models Zhifei Chen, Alina Kondrashina, Ines Greco, Luke F. Gamon, Marianne N. Lund, Linda Giblin, and Michael J. Davies J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.9b01324 • Publication Date (Web): 24 Apr 2019 Downloaded from http://pubs.acs.org on April 24, 2019
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Journal of Agricultural and Food Chemistry
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Effects of Protein-Derived Amino Acid Modification Products Present in Infant Formula on
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Metabolic Function, Oxidative Stress and Intestinal Permeability Using Cell Models
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Zhifei Chen a, Alina Kondrashina b, Ines Greco c, Luke F. Gamon a, Marianne N. Lund a,c, Linda
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Giblin b, and Michael J. Davies a *
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a Department
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Copenhagen, Copenhagen, Denmark
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b
of Biomedical Sciences, Faculty of Health and Medical Sciences, University of
Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland
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c Department
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Denmark
of Food Science, Faculty of Science, University of Copenhagen, Copenhagen,
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Corresponding Author:
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Prof. Michael J. Davies
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Department of Biomedical Sciences, Panum Institute, University of Copenhagen, Blegdamsvej 3,
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Copenhagen 2200, Denmark
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* Phone : +45 23 64 94 45
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E-mail:
[email protected] 22
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT: Proteins present in infant formulas are modified by oxidation and glycation during
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processing. Modified amino acid residues released from proteins may be absorbed in the
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gastrointestinal tract, and pose a health risk to infants. In this study markers of glycation, furosine
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(1.7-3.5 µg mg-1 protein) and Nɛ-(carboxymethyl)lysine (28-81 ng mg-1 protein), were quantitated in
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infant formulas. The effects of these species, and other amino acid modifications, at the levels
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detected in infant formulas, on 3T3-L1 (murine pre-adipocyte) and Caco-2 (human intestinal
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epithelial) cells were assessed. Incubation of 3T3-L1 cells for 48 h with amino acid side-chain
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oxidation and glycation products (1 and 10 µM), resulted in a loss (up to 40 %; p
