Supporting Information for:
Effects of proteins on protein diffusion Yaqiang Wang,1 Conggang Li,1 and Gary J. Pielak1,2,3,*
1
Department of Chemistry, 2Department of Biochemistry and Biophysics, 3Lineberger
Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599.
*To whom correspondence should be addressed: Kenan Laboratories, University of North Carolina, Chapel Hill, NC 27599-3290. Phone: (919)-966-3671. Fax: (919)-843-1580. E-mail:
[email protected].
Supporting Information for Wang et al., page S2
Table S1. Translational and rotational diffusion coefficients for chymotrypsin inhibitor 2 Crowder Crowder
g/L
Viscosity cP
Translation 10-7 cm2/sec
Rotation 107 rad2/sec
R1R2 s-2
Buffer
-
1.0
15.51
4.07
13.8
Glycerol
200
1.5
9.46
2.60
15.1
Glycerol Glycerol
350 420
2.9 3.8
5.17 4.13
1.36 1.07
16.2 17.1
PVP
100
7.6
4.24
2.03
18.4
PVP PVP
200 300
21.5 53.8
1.87 0.95
1.14 0.58
22.1 29.5
Ficoll Ficoll Ficoll
100 200 300
2.5 9.8 24.3
5.33 2.59 1.32
2.49 1.89 1.22
15.6 18.0 20.7
Lysozyme Lysozyme Lysozyme Lysozyme
50 100 200 300
1.1 1.3 1.6 3.9
12.20 5.12 3.86 1.39
2.25 0.65 0.52 -
19.3 33.3 40.3 -
Ovalbumin Ovalbumin Ovalbumin
50 100 200
1.1 1.4 2.0
13.30 9.02 6.76
2.56 1.06 0.82
17.8 23.1 28.8
Ovalbumin
300
4.5
3.14
-
-
BSA BSA BSA BSA
50 100 200 300
1.2 1.5 2.5 4.8
13.22 8.18 5.51 2.66
2.65 1.05 0.64 0.36
17.4 22.3 36.1 52.6
Lysate Lysate
100 200
2.5 3.6
7.07 3.97
1.17 0.58
24.3 38.7
Supporting Information for Wang et al., page S3
Conditions for glycerol, PVP and Ficoll: 50 mM acetate buffer, pH 5.4, 25 oC. Conditions for BSA, ovalalbumin, lysozyme: 200 mM acetate buffer, pH 5.4, 25 oC. The PVP data have been published.1 (1)
Li, C.; Wang, Y.; Pielak, G. J. J. Phys. Chem. B 2009, 113, 13390-13392.
