(ELISA) for the Quantification of Thiram in Lettuce - American

Mar 29, 2001 - Laboratoire de Microbiologie et Sécurité Alimentaire, ESMISAB, Technopôle Brest-Iroise, 29280 Plouzané,. France, and Laboratoire de...
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J. Agric. Food Chem. 2001, 49, 1675−1680

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Development of an Immunoassay (ELISA) for the Quantification of Thiram in Lettuce Anne-Laurence Queffelec,† Frederic Boisde´,‡ Jean-Pierre Larue,† Jean-Pierre Haelters,‡ Bernard Corbel,‡ Daniel Thouvenot,† and Patrice Nodet*,† Laboratoire de Microbiologie et Se´curite´ Alimentaire, ESMISAB, Technopoˆle Brest-Iroise, 29280 Plouzane´, France, and Laboratoire de Chimie He´te´ro-Organique, FRE 2125 CNRS-UBO-MNHM, UFR Sciences et Techniques de Brest, B.P. 809, 29285 Brest Cedex, France

Two competitive immunoassays, a laboratory assay based on microwell plates and a field test based on the use of polystyrene tubes, have been developed for the quantification of thiram in lettuces. Concerning the laboratory assay, the calibration curve for thiram had a linear range of 11 to 90 ng/mL and a detection limit of 5 ng/mL. Precision of the assay presented coefficient of variation values