Article pubs.acs.org/est
Endocrine Disrupting Compounds Affecting Corticosteroid Signaling Pathways in Czech and Swiss Waters: Potential Impact on Fish Petra Macikova,*,† Ksenia J. Groh,‡,§ Adrian A. Ammann,‡ Kristin Schirmer,‡,∥,⊥ and Marc J.-F. Suter*,‡,∥ †
Masaryk University, Faculty of Science, RECETOX, 62500 Brno, Czech Republic Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland § ETH Zürich, Department of Chemistry and Applied Biosciences, 8093 Zürich, Switzerland ∥ ETH Zürich, Department of Environmental Systems Science, 8092 Zürich, Switzerland ⊥ EPF Lausanne, School of Architecture, Civil and Environmental Engineering, 1015 Lausanne, Switzerland ‡
S Supporting Information *
ABSTRACT: This study investigated the occurrence of corticosteroid signaling disruptors in wastewaters and rivers in the Czech Republic and in Switzerland. 36 target compounds were detected using HPLC-MS/MS, with up to 6.4 μg/L for azole antifungals that indirectly affect corticosteroid signaling. Glucocorticoid receptor (GR)-mediated activity was determined using the GR-CALUX bioassay with dexamethasone equivalent concentrations ranging from
