Engineering Translational Resource Allocation Controllers

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Letter

Engineering Translational Resource Allocation Controllers: Mechanistic Models, Design Guidelines, and Potential Biological Implementations Alexander Darlington, Juhyun Kim, José I Jiménez, and Declan G Bates ACS Synth. Biol., Just Accepted Manuscript • DOI: 10.1021/acssynbio.8b00029 • Publication Date (Web): 15 Oct 2018 Downloaded from http://pubs.acs.org on October 16, 2018

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore,

is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

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is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

a

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ACS Synthetic Biology

Disturbance

Process

+ + +

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o-ribo.

Controller

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d

+ -

e

f

ACS Paragon Plus Environment

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e ACS Paragon Plus Environment

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b

Increasing circuit copy number

c

Increasing strength of RBS of 𝒑𝟏 g1,T = g2,T = 10 kL1/kL2 = 100

g1,T = g2,T = 10 kL1/kL2 = 10

g1,T = g2,T = 10 kL1/kL2 = 1

g1,T = g2,T = 10 kL1/kL2 = 0.1

g1,T = g2,T = 10 kL1/kL2 = 0.01

g1,T = g2,T = 100 kL1/kL2 = 100

g1,T = g2,T = 100 kL1/kL2 = 10

g1,T = g2,T = 100 kL1/kL2 = 1

g1,T = g2,T = 100 kL1/kL2 = 0.1

g1,T = g2,T = 100 kL1/kL2 = 0.01

g1,T = g2,T = 500 kL1/kL2 = 100

g1,T = g2,T = 500 kL1/kL2 = 10

g1,T = g2,T = 500 kL1/kL2 = 1

g1,T = g2,T = 500 kL1/kL2 = 0.1

g1,T = g2,T = 500 kL1/kL2 = 0.01


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Engineering Translational Resource Allocation Controllers

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