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Evaluation of biostimulation and bioaugmentation to stimulate RDX degradation in an aerobic groundwater aquifer Mandy M Michalsen, Aaron S King, Rebecca A Rule, Mark E Fuller, Paul B. Hatzinger, Charles W. Condee, Fiona H. Crocker, Karl J. Indest, Carina M. Jung, and Jack D. Istok Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.6b00630 • Publication Date (Web): 14 Jun 2016 Downloaded from http://pubs.acs.org on June 18, 2016
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Environmental Science & Technology
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Evaluation of biostimulation and bioaugmentation to stimulate RDX degradation in an aerobic
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groundwater aquifer.
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Mandy M. Michalsen1*, Aaron S. King2, Rebecca A. Rule2 Mark E. Fuller3, Paul B. Hatzinger3, Charles W.
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Condee3, Fiona H. Crocker1, Karl J. Indest1, Carina M. Jung1 and Jack D. Istok4
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U.S. Army Engineer Research and Development Center, Environmental Laboratory, Vicksburg, MS 39180
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U.S. Army Corps of Engineers, Seattle District, Seattle, WA 98134
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CB&I Federal Services, 17 Princess Road, Lawrenceville, NJ 08648
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School of Civil and Construction Engineering, Oregon State University, Corvallis, OR 97331
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To whom correspondence should be sent: Mandy Michalsen Seattle District USACE 4735 E. Marginal Way S. Seattle, WA 98134 Telephone: 206-764-3324 Fax: 206-764-3706 Email:
[email protected] KEYWORDS: RDX; KTR9; bioaugmentation; groundwater; bioremediation; aerobic
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Supporting Information. Site maps showing well locations and geologic cross-section
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ACS Paragon Plus Environment
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Environmental Science & Technology
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ABSTRACT
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military sites. This study compared in situ RDX degradation rates following bioaugmentation with Gordonia sp.
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strain KTR9 (henceforth KTR9) to rates under biostimulation conditions in an RDX-contaminated aquifer in
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Umatilla, OR. Bioaugmentation was achieved by injecting site groundwater (6000 L) amended with KTR9 cells
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(108 cells mL-1) and low carbon substrate concentrations (
