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Evaluation of the bioavailability and metabolism of nitroderivatives of hydroxytyrosol using Caco-2 and HepG2 human cell models Elena Gallardo, Beatriz Sarriá, José Luis Espartero, José Antonio González-Correa, Laura Bravo, and Raquel Mateos J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b00401 • Publication Date (Web): 26 Feb 2016 Downloaded from http://pubs.acs.org on March 1, 2016
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Evaluation of the Bioavailability and Metabolism of Nitroderivatives of
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Hydroxytyrosol Using Caco-2 and HepG2 Human Cell Models
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Elena Gallardo1,2,3, Beatriz Sarria1, José Luis Espartero2, José Antonio Gonzalez Correa3, Laura
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Bravo-Clemente1, Raquel Mateos1*
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(ICTAN), CSIC, Madrid, Spain; 2Department of Organic and Pharmaceutical Chemistry, Faculty
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of Pharmacy, University of Seville, Spain; 3Department of Pharmacology and Therapeutics,
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School of Medicine, University of Malaga.
Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition
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Running title: Evaluation of the bioavailability and metabolism of nitroderivatives of
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hydroxytyrosol
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*Corresponding author:
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Raquel Mateos Briz (
[email protected])
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Department of Metabolism and Nutrition
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Institute of Food Science, Technology and Nutrition (ICTAN-CSIC). Spanish National Research
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Council (CSIC). C/ José Antonio Nováis 10, 28040 Madrid, Spain.
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Tel. +34.915492300
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Fax: +34.915493627
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Journal of Agricultural and Food Chemistry
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Abstract
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Considering that nitrocatechols present putative effect against Parkinson Disease, the
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absorption and metabolism of nitroderivatives of hydroxytyrosol (HT) were assessed using
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human
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nitrohydroxytyrosyl acetate (NO2HT-A), and ethyl nitrohydroxytyrosyl ether (NO2HT-E) were
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efficiently transferred across human Caco-2 cell monolayers as an intestinal barrier model,
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being NO2HT-A and NO2HT-E better (p
