Fill yourself in on reagents for electrophoresis
In addition to a large list of basic reagents for acrylamide gel elec trophoresis, Eastman Kodak Company offers some with very special properties for special applications. For instance: A cross-linking agent to form solubilizable gel, N,N'Diallyltartardiamide (EASTMAN 1 1 4 4 4 ) . By substituting Ν,Ν'-diallyltartardiamide mole for mole in place of methylenebisacrylamide, acrylamide gels are formed which are soluble in 2 % periodic acid. These gels dissolve in 2 0 to 3 0 minutes at room temperature, or in about 1 0 minutes at 3 7 C. Gels prepared with this reagent take longer to set (about 1 hour for a 7 % gel) than those prepared with methylenebisacrylamide, but otherwise, properties of the gels are similar. [FEBS Letters, 7, No. 3 , 2 9 3 (1970).] A fluorescent dye that stains at neutral p H . 8-Anilino1-Naphthalenesulfonic Acid Magnesium Salt (EAST MAN 1 0 9 9 0 ) . This dye rapidly binds with proteins at neutral pH producing a yellow fluorescence. Since acid condi tions are not required for staining and destaining, protein denaturation is minimal. This visualization technique is useful for preparative electrophoresis, where further study or analysis of the purified pro teins is desirable. [Ana/. Biochem., 30, 3 9 1 ( 1 9 6 9 ) and Neurospora Newsletter, No. 1 5 , 1 6 (December 1969).] A dye for differential staining of DNA and RNA. 1 Ethy!-2-[3-(l-ethylnaphtho[l,2d]-thiazolin-2-ylidene)-2-methylpropenyl]-naphtho[l,2d]-thiazolium Bromide (EASTMAN 2 7 1 8 ) .
This dye is reported to stain RNA bluish-purple, DNA blue, protein red, and is also useful for staining acid polysaccharides. Because of the versatility of this dye by virtue of its differential staining ability, t h e reagent has been called "Stains-All." [J. Mo/. Biol., 41, 1 3 9 ( 1 9 6 9 ) . ] The dye has also been used for estimating lipopolysaccharides. [FEBS Letters, 16, 343 (1971).] • A dye to stain gels for quantitative densitometric analysis. Fast Green FCF (Cert.) (EASTMAN C 8 6 8 6 ) . Fast Green FCF is reported to stain a number of acidic or basic proteins for quantitation by densitom etry after electrophoretic separation. The stain obeys Beer's law between 150 and 2 0 0 ^g of protein on 6 m m gels and has a sensitivity comparable to amido black, yet shows no evidence of metachromacy, a drawback of amido black. [Ana/. Biochem., 35, 3 5 9 (1970).] Don't overlook EASTMAN Organic Chemicals for t h e basics, either . . . an assortment of initiators, stains, buffer components, and specially purified monomers. For a complete list, including prices and package sizes, use the coupon below to request Kodak Publication No. J J - 1 1 , Reagents for Acrylamide Gel Electrophoresis. To order these or any EASTMAN Organic Chemical, contact: CURTIN FISHER NORTH-STRONG
PREISER SARGENT-WELCH VWR SCIENTIFIC (EAST)
Dept. 412L Eastman Organic Chemicals Eastman Kodak Company Rochester, Ν. Υ. 14650 •
Please send a copy of JJ-11:
Name
Kodak
Address City State
Zip
12-211
Dec. 18, 1972 C&EN
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