FRET Conformational Analysis of Calmodulin Binding to Nitric Oxide

Oct 24, 2008 - bridge, UK). The RO value was determined ...... Marletta, M. A., and Stevens-Truss, R. (2005) Calcium-binding sites of calmodulin and e...
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12006

Biochemistry 2008, 47, 12006–12017

FRET Conformational Analysis of Calmodulin Binding to Nitric Oxide Synthase Peptides and Enzymes† Donald E. Spratt, Valentina Taiakina, Michael Palmer, and J. Guy Guillemette* Department of Chemistry, UniVersity of Waterloo, Waterloo, Ontario N2L 3G1, Canada ReceiVed July 29, 2008; ReVised Manuscript ReceiVed September 10, 2008

Calmodulin (CaM) is a ubiquitous Ca2+-sensor protein that binds and activates the nitric oxide synthase (NOS) enzymes. We have used fluorescence resonance energy transfer (FRET) to examine the conformational transitions of CaM induced by its binding to synthetic nitric oxide synthase (NOS) CaMbinding domain peptides and full length heme-free constitutive NOS (cNOS) enzymes over a range of physiologically relevant free Ca2+ concentrations. We demonstrate for the first time that the domains of CaM collapse when associated with Ca2+-independent inducible NOS CaM-binding domain, similar to the previously solved crystal structures of CaM bound to the Ca2+-dependent cNOS peptides. We show that the association of CaM is not detectable with the cNOS peptides at low free Ca2+ concentrations (