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Gene Expression Patterns are Altered in Athymic Mice and Metabolic Syndrome Factors are Reduced in C57BL/6J Mice Fed High-fat Diets Supplemented with Soy Isoflavones Ting Luo, Sarah M. Snyder, Bingxin Zhao, Debra K. Sullivan, Jill Hamilton-Reeves, Gregory Guthrie, Marie-Louise Ricketts, Kathleen T. Shiverick, and Neil Shay J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b03401 • Publication Date (Web): 21 Sep 2016 Downloaded from http://pubs.acs.org on September 27, 2016
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Gene Expression Patterns are Altered in Athymic Mice and Metabolic
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Syndrome Factors are Reduced in C57BL/6J Mice Fed High-fat Diets
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Supplemented with Soy Isoflavones
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Luo T†, Snyder SM†, Zhao B†, Sullivan DK‡, Hamilton-Reeves J‡, Guthrie G§, Ricketts MLΔ,
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Shiverick KT∥ and Shay N†*
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Food Science and Technology, Oregon State University, Corvallis 97330, USA
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Dietetics and Nutrition, Kansas University Medical Center, Kansas City, 66160, USA
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Baylor College of Medicine, Houston, 77030, USA
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Agriculture, Nutrition and Veterinary Sciences, University of Nevada, Reno, 89557, USA
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Pharmacology, University of Florida College of Medicine, Gainesville 32610, USA
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Running title: Gene expression is altered and MetS symptoms are reduced by soy
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isoflavone supplementation
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ACS Paragon Plus Environment
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Page 3 of 43
Journal of Agricultural and Food Chemistry
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ABSTRACT:
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Soy isoflavones exert beneficial health effects, however, their potential to ameliorate
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conditions associated with the metabolic syndrome (MetS) has not been studied in detail. In
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vitro and in vivo models were used to determine the effect of isoflavones on lipid metabolism,
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inflammation, and oxidative stress. In nude mice, consumption of Novasoy (NS) increased
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cholesterol and lipid metabolism gene expression, including Scd-1 (27.7-fold), Cyp4a14
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(35.2-fold), and Cyp4a10 (9.5-fold); and reduced anti-inflammatory genes, including Cebpd
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(16.4-fold). A high-fat (HF) diet containing 0.4% (w/w) NS for 10 weeks significantly reduced
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percent weight gain (74.6%±2.5% vs. 68.6%±3.5%) and hepatic lipid accumulation
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(20%±1.2% vs. 27%±1.5%), compared to HF alone (p
