Gene Expression Patterns Are Altered in Athymic Mice and Metabolic

Sep 21, 2016 - Diets rich in polyunsaturated fat, low glycemic index foods, and foods with a high fiber content are recommended to reduce MetS...
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Gene Expression Patterns are Altered in Athymic Mice and Metabolic Syndrome Factors are Reduced in C57BL/6J Mice Fed High-fat Diets Supplemented with Soy Isoflavones Ting Luo, Sarah M. Snyder, Bingxin Zhao, Debra K. Sullivan, Jill Hamilton-Reeves, Gregory Guthrie, Marie-Louise Ricketts, Kathleen T. Shiverick, and Neil Shay J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b03401 • Publication Date (Web): 21 Sep 2016 Downloaded from http://pubs.acs.org on September 27, 2016

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Journal of Agricultural and Food Chemistry

ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

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Gene Expression Patterns are Altered in Athymic Mice and Metabolic

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Syndrome Factors are Reduced in C57BL/6J Mice Fed High-fat Diets

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Supplemented with Soy Isoflavones

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Luo T†, Snyder SM†, Zhao B†, Sullivan DK‡, Hamilton-Reeves J‡, Guthrie G§, Ricketts MLΔ,

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Shiverick KT∥ and Shay N†*

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Food Science and Technology, Oregon State University, Corvallis 97330, USA



Dietetics and Nutrition, Kansas University Medical Center, Kansas City, 66160, USA

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Baylor College of Medicine, Houston, 77030, USA

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Agriculture, Nutrition and Veterinary Sciences, University of Nevada, Reno, 89557, USA



Pharmacology, University of Florida College of Medicine, Gainesville 32610, USA

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Running title: Gene expression is altered and MetS symptoms are reduced by soy

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isoflavone supplementation

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ACS Paragon Plus Environment

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Journal of Agricultural and Food Chemistry

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ABSTRACT:

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Soy isoflavones exert beneficial health effects, however, their potential to ameliorate

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conditions associated with the metabolic syndrome (MetS) has not been studied in detail. In

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vitro and in vivo models were used to determine the effect of isoflavones on lipid metabolism,

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inflammation, and oxidative stress. In nude mice, consumption of Novasoy (NS) increased

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cholesterol and lipid metabolism gene expression, including Scd-1 (27.7-fold), Cyp4a14

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(35.2-fold), and Cyp4a10 (9.5-fold); and reduced anti-inflammatory genes, including Cebpd

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(16.4-fold). A high-fat (HF) diet containing 0.4% (w/w) NS for 10 weeks significantly reduced

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percent weight gain (74.6%±2.5% vs. 68.6%±3.5%) and hepatic lipid accumulation

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(20%±1.2% vs. 27%±1.5%), compared to HF alone (p