Subscriber access provided by EAST TENNESSEE STATE UNIV
Article
Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol Ying-Hsuan Lin, Maiko Arashiro, Phillip W. Clapp, Tianqu Cui, Kenneth G. Sexton, William Vizuete, Avram Gold, Ilona Jaspers, Rebecca C Fry, and Jason Douglas Surratt Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b01967 • Publication Date (Web): 21 Jun 2017 Downloaded from http://pubs.acs.org on June 25, 2017
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
Environmental Science & Technology is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 38
Environmental Science & Technology
1
Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived
2
Secondary Organic Aerosol
3 4
Ying-Hsuan Lin,1,a,* Maiko Arashiro,1,b Phillip W. Clapp,2 Tianqu Cui,1 Kenneth G. Sexton,1
5
William Vizuete,1 Avram Gold,1 Ilona Jaspers,1,2,3 Rebecca C. Fry,1 and Jason D. Surratt1, *
6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
1
Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States 2 Center for Environmental Medicine, Asthma and Lung Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States 3 Department of Pediatrics, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States a Current address: Department of Environmental Sciences, Environmental Toxicology Graduate Program, University of California, Riverside, California 92521, United States b Current address: Department of Environmental Studies, Dickinson College, Carlisle, Philadelphia 17013, United States *
To whom correspondence should be addressed. Tel.: (951)-827-3785. Fax: (951)-827-4652. Email:
[email protected] (Y.-H. L) Tel.: (919)-966-0470. Fax: (919)-966-7911. Email:
[email protected] (J.D.S.)
23 24 25 26 27 28 29 30 31
ACS Paragon Plus Environment
Environmental Science & Technology
32
TOC Art
33
ACS Paragon Plus Environment
Page 2 of 38
Page 3 of 38
Environmental Science & Technology
34
Abstract
35
Secondary organic aerosol (SOA) derived from the photochemical oxidation of isoprene
36
contributes a substantial mass fraction to atmospheric fine particulate matter (PM2.5). The
37
formation of isoprene SOA is influenced largely by anthropogenic emissions through multiphase
38
chemistry of its multi-generational oxidation products. Considering the abundance of isoprene
39
SOA in the troposphere, understanding mechanisms of adverse health effects through inhalation
40
exposure is critical to mitigating its potential impact on public health. In this study, we assessed
41
the effects of isoprene SOA on gene expression in human airway epithelial cells (BEAS-2B)
42
through an air-liquid interface exposure. Gene expression profiling of 84 oxidative stress and 249
43
inflammation-associated human genes was performed. Our results show that the expression
44
levels of 29 genes were significantly altered upon isoprene SOA exposure under non-cytotoxic
45
conditions (p
