Genomics technology revolutionizes environmental monitoring

Genomics technology revolutionizes environmental monitoring. Janet Pelley. Environ. Sci. Technol. , 2000, 34 (7), pp 164A–165A. DOI: 10.1021/es00320...
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Environmental News appropriated last year and falls far short of the $40 million Congress authorized for 2001 in the Hydrogen Futures Act. This latest funding request is unsupportable, given that DOE highly rated hydrogen's carbon displacement level, the measure used to determine which climate change technologies should receive funding, said Karen Miller, director of the National Hydrogen Association, a nonprofit group representing both industry and researchers. She said her association plans to lobby hard for a higher budget. Thanks to the support hydrogen technology has on Capitol Hill, its funding levels are likely to be buttressed in the next stage of the appropriations process that begins in May, said Robert Dixon, acting deputy assistant administrator in DOE's Office of Power Technologies. Dixon stressed that some additional hydrogen research may be conducted through other programs, but acknowledged that to date, hydrogen is not covered by the $27 million that DOE has requested for research into ultraclean fuels for transportation Hydrogen technology's promise drew attention just weeks after the agency's budget was announced in February, when researchers from two DOE labs reported a major breakthrough that

The budget for hydrogen research, which resulted in a major breakthrough in generating hydrogen from the algae in pond scum this year, is slated to be cut in 2001.

could enable algae to be converted into hydrogen for use in fueling vehicles. "Auto companies have publicly stated that if certain technical issues with hydrogen were solved, they would not be looking for other alternate fuels," Miller pointed out. As a whole, the request for solar and renewable resource technologies—of which hydrogen research is a part—is up by 28% to $456.6 million. Photovoltaics are up for a 24% increase to $82 million, and wind energy is slotted for a 56% boost to $50.5 million. Efforts to harness energy from biomass, a major initiative an-

nounced by President Clinton last year, got a 39% increase to $174 million in the 2001 request. Another big winner is carbon sequestration research, which would receive $42.6 million—49% more than last year—for efforts like separating C0 2 from industrial process streams and capturing it for permanent storage in geological formations. And the agency's security and emergency operations program garnered the largest monetary increase in 2001 of $56.2 million in response to the highly publicized spy scandal at Los Alamos National Laboratorvlast year But DOE's $6 3 billion reauest for its environmental management budget which pavs for the agenev's remediation Droiects earned a modest 2% increase It is "way too early" to tell what the actual appropriations will be since they are unlikely to be finalized before June, said a member of the Senate Appropriations Committee's Energy and Water Resources Subcommittee, which is in charge of DOE's budget appropriations. But elected officials in both political parties generally pay particular attention to solar and renewable-energy technologies during DOE's appropriation hearings arid any area favored with, an increase of than 20% is also likely to be fully examined he said KELLYN S. BETTS

Genomics technology revolutionizes environmental monitoring A pilot program launched last year by Environment Canada, the Canadian counterpart to EPA, to apply biomolecular tools to environmental problems is yielding new information on ways to monitor pollutants and conserve wildlife. Researchers agree that the program may ultimately change the way the environment is regulated, according to Terry Mclntyre, acting chief of Environment Canada's Bioproducts and Processes Application division At the annual national science meeting of Environment Canada's Ecological Monitoring and

Assessment Network held in Toronto, Ontario, in January, Mclntyre described some of the projects undertaken through the program over the past year that are using what is broadly known as genomics technology, the study of how genes are organized and function. One promising technique involves the detection of hydrocarbon-contaminated soil using naturally occurring bacteria. Explaining the technique John Lawrence a research scientist with the National Water Research Institute in Saskatoon Saskatchewan and colleague

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LjiiarlGS vjiecij a. research onicer with the Biotechnology Research Institute in Montreal, Quebec, said they had identified 16 different bacterial genes that code for enzymes that degrade hydrocarbons, and Greer annealed the gene sequences onto a 0.5-in glass chip. Lawrence and Greer have used the genes arrayed on the chip to monitor bacterial communities upstream and downstream of areas contaminated with petroleum products. The researchers detect hydrocarbondegrading genes by extracting

and labeling DNA from native bacteria populations in soil samples and analyzing it on Greer's chip. Using a microscope hooked up to a computer, the researchers look for DNA that sticks to the chip to show that microbes that degrade hydrocarbons are present in the soil sample. "The molecular tools give you a direct way of measuring biodiversity and allow you to monitor how it changes when you threaten it, and how it comes back to health after cleanup," Greer said. He predicts that environmental regulations will change to incorporate the new technology because it is more sensitive and sophisticated than current technology for monitoring pollutants and evaluating risk. Other research being conducted by James Quinn, associate professor of biology at McMaster University in Hamilton, Ontario, shows that herring gulls could be used as "the canary in the coal mine for early detection of mutagens in the environment". Quinn has demonstrated that the frequency of mutations in minisatellite DNA, a repetitive section of DNA that is prone to mutations, is highest in herring gulls that live near steel mills in the Great Lakes. The inheritable mutations are proportional to the rate of mutation in DNA sequences that code for proteins that affect the gulls' survival and reproduction he said Quinn's next step is to determine which of three suspects— heavy metals, polychlorinated biphenyls, or polycyclic aromatic hydrocarbons—are causing the mutations. "The intention is to measure mutation rates in gulls in Hamilton Harbor near the steel mills and compare them to mutation rates after the harbor is cleaned up," he said. Ultimately, mutation rates in populations of gulls could be used to detect the presence of mutagens in the environment before any outward signs such disease are ob~ served he said The research could lead to chancres in environmental regulations if for in-

stance, mutagens were found to originate on particulates from smokestacks, Quinn suggested. "Biomolecular tools have the potential to revolutionize wildlife conservation," said Kathy Dickson, senior waterfowl biologist with the Canadian Wildlife Service. She cited research on tracking and identifying migratory birds. Samples of feathers, eggshells, and blood have provided genetic markers that have helped delineate subpopulations of ducks and track them between their wintering and breeding grounds. Compared to banding and recapturing of birds "the biomolecular tools are more costeffective and allow us to answer questions we had no way of askint? in the past " Dickson said "Biomolecular techniques as a whole have tremendous potential for improving environmental

monitoring, but we have to balance their rapid introduction with careful consideration of their impacts on society and the environment," cautioned Mclntyre. The new techniques could, for instance, reveal that certain genes predispose women of child-bearing age to be more sensitive to certain pollutants, and exposure limits could be reduced accordingly, he said. The technology could also raise genetic privacy issues if certain individuals prove to be more sensitive to the pollutants because of their genetic makeup Mclntyre is trying to catalyze the formation of a national Canadian environmental genomics network and has hired a consultant to inve'stigate how to inte environmental concerns into the projects that Fnvirnnment Canada is fundinp JANET PELLEY

Sustainability New sustainability benchmark takes aim at the GDP A pilot environmental index with aspirations to challenge the Gross Domestic Product (GDP) as the preeminent measure of society's well-being made its debut in January. Since then, thousands of people worldwide in government, academia, industry, and nongovernmental organizations have downloaded copies of it. "Everyone who has studied the subject recognizes that the GDP isn't adequate," said Jim Salzman, a law professor at American University. The GDP focuses on economic growth, so it increases as the percentage of people suffering from cancer and the frequency of oil spills rises because such environmentally undesirable things generate economic activity, he explained. Measures of how well countries maintain the health of their environments will play a key role in the coming century, according to a National Academy of Engineering report published last year, and a number of high-profile efforts are under way to create such environmental indicators. What sets the new environmental sustainability index (ESI) apart is its sole focus on environmental matters and its ability to compare the performance of different countries over time, according to its developers. It was produced as a collaboration between researchers at Columbia and Yale Universities and members of the World Economic Forum's Global Leaders for Tomorrow, an international group with members in business, academia, government, and environmental groups. "We view the ESI as playing a role similar to a cumulative grade point average," said Daniel Esty, director of the Yale Center for Environmental Law and Policy, who also directed the project to build the index. The pilot index is available on the Web site maintained by Columbia University's Center for International Earth Science Information Network (www. ciesin.org). The authors welcome criticism, Esty said, and they hope to have a final version available by the end of the year. —KELLYN S. BETTS

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