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J. Agric. Food Chem. 1998, 46, 1670−1676
Hapten Synthesis for a Monoclonal Antibody Based ELISA for Deltamethrin Anne-Laurence Queffelec,†,‡ Patrice Nodet,*,† Jean-Pierre Haelters,‡ Daniel Thouvenot,† and Bernard Corbel‡ Laboratoire de Microbiologie et Se´curite´ Alimentaire, ESMISAB, Technopoˆle Brest-Iroise, 29280 Plouzane´, France, and Laboratoire de Chimie He´te´ro-Organique, UFR Sciences et Techniques de Brest, B.P. 809, 29285 Brest Cedex, France
An enzyme-linked immunosorbent assay (ELISA) was developed for the insecticide deltamethrin. Two haptens were synthesized: the first one was a derivative of the whole molecule with a spacer arm bound to the aromatic ring; the second one was a derivative of deltamethric acid. Twelve monoclonal antibodies were obtained. A competitive ELISA using monoclonal antibody Del 01 yielded the most sensitive assay. The IC50 value for deltamethrin was estimated to be 0.5 µg mL-1 with a detection limit of 0.08 µg mL-1. Monoclonal antibody Del 01 seems to be deltamethrin specific since
