Heterocyclic Amines in Fresh and Processed Meat Products

Processed meats (bratwurst, fresh pork sausage, Italian sausage, and light smoked sausage) were evaluated for heterocyclic amine content. Eye round st...
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J. Agric. Food Chem. 1998, 46, 4680−4687

Heterocyclic Amines in Fresh and Processed Meat Products Basira G. Abdulkarim and J. Scott Smith* Department of Animal Sciences and Industry, 208 Call Hall, Kansas State University, Manhattan, Kansas 66506

Heterocyclic amines (HCAs), potent mutagens/carcinogens, are pyrolysis products formed during the cooking of meat and fish. Processed meats (bratwurst, fresh pork sausage, Italian sausage, and light smoked sausage) were evaluated for heterocyclic amine content. Eye round steak and ground beef with two fat levels (5 and 15%) also were evaluated. Meat samples fried at 150, 190, and 230 °C or grilled at 200 and 240 °C were analyzed by HPLC using ultraviolet and fluorescence detection. Both the interior and external surfaces of the patties were evaluated. The crust of the Italian sausage showed the highest level of heterocyclic amine 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (3.44 ng/g), whereas that of smoked sausage showed the highest levels of 2-aminomethyl-6-phenylimidazo[4,5-b]pyridine (5.83 ng/g), 1-methyl-9H-pyrido[3,4-b]indole (10.6 ng/g), and 9H-pyrido[3,4-b]-indole (2.51 ng/g). The 5% fat beef patties showed higher HCA content than the 15% fat beef patties. Keywords: Fresh; processed; meat products; heterocyclic amines; MeIQx; PhIP INTRODUCTION

Heterocyclic amines (HCAs) are the main mutagenic and probably carcinogenic substances produced in cooked muscle meat, specifically beef, pork, mutton, chicken, and fish. These compounds are mutagenic in the Ames/ Salmonella assay (Sugimura, 1985). Several long-term animal studies on mice, rats, and nonhuman primates have suggested a correlation between HCA consumption and cancer (Layton et al., 1995). Furthermore, epidemiological studies have suggested a relationship between high consumption of meat and fish and different types of human cancer such as pancreatic, colorectal, and urothelial cancer (Gerhardsson de Verdier, 1995). Physical variables such as temperature, time, and method of cooking significantly affect the mutagenic activity of cooked meat. Cooking temperature is the most important factor (Sugimura and Sato, 1983). Studies show a marked decrease in mutagenic activity of meat when it is fried at lower temperatures (Skog et al., 1995). Although all cooked meat has some mutagenic activity, meat cooked at e150 °C to rare or medium-rare doneness showed fewer mutagens at lower content than meat cooked to well-done (g150 °C) (Hatch et al., 1988). Moreover, the surface of well-done charcoalbroiled steak contained much higher levels of HCAs than that of boiled beef. Several studies have postulated that the products of the Maillard reaction, such as pyridines or pyrazines formed by Strecker degradation, undergo an aldol-type condensation (Ja¨gerstad et al., 1990). The resulting vinylpyridines or vinylpyrazines go through a conjugation addition with creatinine. The reaction is completed by ring closure, water elimination, and desaturation to give imidazoquinolines, 2-amino-3-methylimidazo[4,5f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]* Author to whom correspondence should be addressed [telephone (785) 532-1219; fax (785) 532-5681; e-mail jsschem@ ksu.edu].

quinoline (MeIQ); imidazoquinoxalines, 2-amino-3,8dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx); and the imidazopyridine 2-amino-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). The other suggested route for the formation of these mutagens is condensation of an aldehyde with creatinine, followed by combination with pyrazines to form imidazoquinoline or imidazoquinoxaline compounds (Jones and Weisburger, 1988). Cyclization and water elimination from a creatine molecule provide the essential 2-amino-3-methylimidazole part of the HCA molecule. This reaction occurs readily if the temperature of the meat rises above 100 °C. Several studies have suggested that these mutagens form in different types of meats. Layton et al. (1995) listed the contents of HCAs in a variety of cooked muscle foods, such as ground beef, beef extract, beef steak, chicken, lamb, pork, and fish, and also in nonmuscle foods, such as mushrooms and Worcestershire sauce. Stavric et al. (1997) surveyed for the presence of HCAs in processed, ready-to-eat meat products such as turkey breast, salami, and chicken loaf. They concluded that no serious health risk will be encountered from the consumption of such meat products due to undetectable levels of HCAs. Sinha et al. (1998) analyzed the HCA content of different pork products cooked by frying, oven-broiling, boiling, or grilling/barbecuing to different degrees of doneness. Their results indicated that welland very well-done oven-broiled bacon showed the highest levels of PhIP, whereas very well-done pan-fried pork chops contained the highest level of MeIQx. No research has evaluated the levels of HCAs in a wide variety of processed meat products, including cured/ fermented meat products. Therefore, this study was undertaken to determine the level of HCAs in bologna, summer sausage, ham, bratwurst, fresh pork sausage, smoked sausage, Italian sausage, and eye round steak and to investigate the effect of fat content (5 and 15%) in ground beef on the levels of HCA formation.

10.1021/jf980175g CCC: $15.00 © 1998 American Chemical Society Published on Web 10/17/1998

Heterocyclic Amines in Meat MATERIALS AND METHODS Meat Samples. Bologna, summer sausage, ham, bratwurst (20% fat), fresh pork sausage (17% fat), Italian sausage (15% fat), ground beef (5% and 15% fat), and eye round steak were obtained from the Kansas State University Meat Laboratory. Light smoked sausage (14% fat) was purchased from a local supermarket. Ingredients of the bratwurst were pork, water, salt, corn syrup, dextrose, spices, flavoring, and monosodium glutamate. The light smoked sausage was made of skinless turkey, pork, water, beef, corn syrup, salt, natural spices, dextrose, monosodium glutamate, ascorbic acid, flavorings, and sodium nitrite. The fresh pork sausage contained salt and spices, whereas the Italian sausage contained pork, salt, caraway seeds, garlic powder, and other spices. Chemicals. All chemicals and solvents were of HPLC grade. Deionized water was used throughout the experimental procedures. Standards for polar HCAs (IQ, MeIQ, MeIQx, 4,8DiMeIQx, and PhIP) and nonpolar HCAs (Trp-p-1, Trp-p-2) and β-carbolines, harman and norharman, were obtained from Toronto Research Chemicals (Toronto, Canada). Extrelut extraction cartridges (20 mL) and refill material were obtained from VWR Scientific Products (Chicago, IL). Bond Elut propylsulfonic silica (PRS) (500 mg) cartridges, and C-18 (100 and 500 mg) cartridges were obtained from Varian Sample Preparation Products (Harbor City, CA). Cartridge coupling adaptors were obtained from Supelco (Bellefonte, PA). Sample Preparation and Cooking Conditions. The various processed meat samples (precooked and raw) were allowed to reach room temperature (20 °C). The cured/ fermented meat samples, bologna, ham, and summer sausage, were sampled and analyzed for HCA content. Raw meat samples were formed into 100 g patties, 1.5 cm thick and 10 cm in diameter, which were fried in a thermostat-controlled Teflon-coated electric griddle (Presto, model 073202) at 150 °C for 6 min, at 190 °C for 10 min, or at 230 °C for 14 min. The smoked sausages were fried by rolling them in the griddle at the same temperatures and times of cooking as the raw meat patties. These samples were also analyzed for HCA content after being charcoal grilled at two temperatures and times, 240 °C for 7 min or 200 °C for 12 min. The temperature of the grill was monitored with a 20-gauge hypodermic probetype thermocouple connected to a Doric temperature recorder (Trendicator 410A, San Diego, CA). This device was also used to measure the internal temperature of the meat patties by inserting the probe into the geometric center of the patty at a 45° angle. For each treatment, three meat samples were cooked (fried or barbecued) at the same time. All of the cooked meat samples were wrapped in plastic wrap and kept frozen until analyzed. Solid-Phase Extraction of Samples. Before analyses, each sample was divided equally in random fashion without bias to heavily browned areas. The brown surfaces (1-2 mm thickness) of identically treated samples were removed with a scalpel. Next, the scrapes of the crusts were pooled and used for analyses after grinding with a Micro-Mill. The remaining halves of the identically treated samples were pooled and used for whole sample analysis. With the precooked sausages, samples from the product’s skin were taken after the casing was removed. Sample cleanup and HCA extraction were based on the method described by Gross and Gru¨ter (1992). At the point of analyses, 3.00 g samples from cooked and precooked meat products were homogenized in 20.0 mL of 1.0 M sodium hydroxide using a commercial Waring blender for 3 min at medium speed. The homogenates were then mixed thoroughly with Extrelut refill material and loaded to empty Extrelut columns. Bond Elut PRS cartridges were fitted with coupling adapters and conditioned with 5 mL of dichloromethane (DCM), and another 2 mL of DCM was pipetted into each cartridge. A Supelco Visiprep SPE vacuum manifold was used for manipulation of eluent flow rate with the sample solid-phase extraction cartridges. To elute HCAs from the Extrelut columns to PRS cartridges, 60 mL of dichloromethane was used. The PRS cartridges were dried for 4 min under maximum vacuum (15

J. Agric. Food Chem., Vol. 46, No. 11, 1998 4681 in. of Hg) and then rinsed successively at 1.5 mL/min with 6 mL of 0.1 M hydrochloric acid, 15 mL of methanol/0.1 M hydrochloric acid (45:55 v/v), and 2 mL of water. These three washes, containing nonpolar amines, were collected into empty Extrelut columns for further extraction. The polar amines were still bonded on the PRS cartridges. Bond Elut C-18 cartridges (100 mg) were used to concentrate the polar amines. These cartridges first were conditioned with 1 mL of methanol, followed by 10 mL of water, and then they were coupled to the PRS cartridges that contained the polar amines. The HCAs were eluted from the PRS cartridge to the C-18 cartridge with 20 mL of 0.5 M ammonium acetate (adjusted to pH 8.0 with concentrated ammonium hydroxide), and the PRS cartridges were discarded. The C-18 cartridges were rinsed with 2 mL of water and dried by applying strong positive nitrogen pressure. The adsorbed polar amines were eluted into microvials using 0.8 mL of methanol-concentrated ammonium hydroxide solution (9:1 v/v). The eluates containing the nonpolar amines were neutralized by adding 0.5 mL of concentrated ammonium hydroxide and diluted with water to