Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching

Jun 2, 2010 - repair and contains a PARP-like zinc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet the...
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Biochemistry 2010, 49, 6165–6176 6165 DOI: 10.1021/bi100503w

Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching between Two DNA-Bound States†,‡ )

Elizabeth Cotner-Gohara,§,# In-Kwon Kim,§,# Michal Hammel, John A. Tainer,^,r Alan E. Tomkinson,@ and Tom Ellenberger*,§ §

)

Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, ^Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, rDepartment of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, and @University of Maryland School of Medicine, Baltimore, Maryland 21201 #These authors contributed equally to this work. Received April 2, 2010; Revised Manuscript Received May 14, 2010 ABSTRACT: Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and

repair and contains a PARP-like zinc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small-angle X-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) forms a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick sensing and the transition from nick sensing by the ZnF to nick joining by the catalytic core. The collective results support a “jackknife model” in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.

DNA ligase III is a vertebrate-specific protein functioning in DNA replication and repair pathways, including nucleotide excision repair, base excision repair, and single-strand break repair, plus mitochondrial replication and repair (1). DNA ligase III is furthermore implicated in the repair of DNA double-strand breaks when nonhomologous end joining (NHEJ)1 activity is compromised (2). Upregulated ligase III expression in chronic myeloid leukemia cells, with concomitant decreases in the level of expression of the NHEJ proteins DNA ligase IV and Artemis, may promote cell survival and disease progression, raising the possibility of selectively inhibiting ligase III as a cancer treatment (3). † This work was supported in part by National Institutes of Health (NIH) Grant 5R01 GM052504 (T.E.) and The Structural Cell Biology of DNA Repair Program (P01 CA92584 to A.E.T., T.E., and J.A.T.). Funding for the SIBYLS beamline was provided in part by the Offices of Science and Biological and Environmental Research, U.S. Department of Energy, under Contract DE-AC02-05CH11231. This work includes research conducted at the Northeastern Collaborative Access Team beamlines of the Advanced Photon Source, supported by Grant RR15301 from the National Center for Research Resources at the National Institutes of Health. Use of the Advanced Photon Source is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract DE-AC02-06CH11357. ‡ Coordinates have been deposited with the Protein Data Bank as entry 3L2P. *To whom correspondence should be addressed. Phone: (314) 3620287. Fax: (314) 362-4432. E-mail: [email protected]. 1 Abbreviations: LigIII, DNA ligase III; NTase, nucleotidyl transferase domain; OBD, oligonucleotide binding domain; DBD, DNA binding domain; ZnF, zinc finger domain; NHEJ, nonhomologous end joining; BRCT, BRCA1-related C-terminal domain; LigI, DNA ligase I; LigIV, DNA ligase IV; SAXS, small-angle X-ray scattering; PARP1, poly(ADP-ribose) polymerase I; rmsd, root-mean-square deviation.

Besides repairing nuclear DNA, ligase III is the only mitochondrial DNA ligase in which it functions in DNA repair and replication. Three DNA ligase III isoforms are generated by alternative mRNA splicing and translation initiation, and expression of one or more of these is essential for the viability of mammalian cells and animals (4). The LigIIIR isoform interacts with XRCC1 through a C-terminal BRCA1-related C-terminal (BRCT) domain, and this protein complex functions in a variety of DNA repair pathways, most prominently in the repair of DNA singlestrand breaks (5, 6). LigIIIβ lacks the C-terminal BRCT domain (6, 7) and is expressed only in the male germ line where it presumably repairs DNA strand breaks during meiotic recombination (7, 8). The mitochondrial DNA ligase III (mtLigIII) isoform has an N-terminal mitochondrial localization sequence besides the C-terminal BRCT interaction domain. However, XRCC1 is absent from mitochondria, and mtLigIII appears to function alone in mitochondrial DNA maintenance (9, 10). Besides LigIII, two other DNA ligases are expressed in mammalian cells. DNA ligase I (LigI) is an essential enzyme that repairs Okazaki fragments during DNA replication and also functions in long patch base excision repair. DNA ligase IV (LigIV) has specialized functions in the repair of DNA doublestrand breaks by the NHEJ pathway, and in the rearrangement of immunoglobulin genes (1, 11). All three mammalian DNA ligases contain a homologous catalytic core, consisting of two domains that are structurally conserved in prokaryotic DNA ligases and other members of a superfamily of nucleotidyl transferases that includes mRNA-capping enzymes and RNA

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ligases (12). Additional N- and C-terminal regions flanking the catalytic core of mammalian DNA ligases provide other functions, including interactions with other proteins that dictate the subcellular localization of each enzyme. The nucleotidyl transferase (NTase) and OB-fold (OBD) domains comprise the catalytic core of DNA ligases that harbors essential residues participating in a three-step DNA end joining reaction (1). A third, noncatalytic domain located immediately N-terminal to the catalytic core of all three mammalian DNA ligases (13) extends the DNA interaction surface of these enzymes. This DNA binding domain (DBD) is essential for DNA recognition and nick joining functions of human LigI (14) and LigIII (15). A crystal structure of LigI bound to a nicked DNA revealed that the DBD and the adjoining NTase and OBD domains form a compact, ring-shaped structure that sequesters the ends of the nicked strand in the active site (14). At present, it is unknown if this protein architecture is conserved in the other mammalian DNA ligases and how structural modifications specific to each enzyme may contribute to their different biological functions. In particular, the LigIII polypeptides are distinguished from the other human DNA ligases by the presence of an N-terminal zinc finger (ZnF) domain that binds cooperatively in conjunction with the adjacent DBD to nicks and gaps in the backbone of duplex DNA (15). This DNA nick sensing by the ZnF evidently contributes to substrate selection and increases the catalytic efficiency of nick joining by DNA ligase III (15-18). Furthermore, the ZnF has a profound effect on stimulating the intermolecular ligation of two DNAs (15), an activity likely relevant to the involvement of LigIIIR/XRCC1 in the back-up pathway of nonhomologous end joining (2). The DNA ligase III ZnF is structurally related to the two N-terminal zinc finger domains of poly(ADP-ribose) polymerase (16). Although a study of the LigIII ZnF domain by NMR identified residues in a β-hairpin motif that are strongly perturbed by the addition of DNA (17), the molecular mechanisms by which DNA nick sensing and catalytic activity are enhanced by the ZnF of LigIII are unknown. To characterize the DNA interaction and structure of human ligase III, we combined X-ray crystal structural analysis with small-angle X-ray scattering (SAXS) to analyze solution architectures and flexibility. These experiments suggest how DNA ligase III interactions and conformational changes contribute to DNA substrate selection and end joining activities. This multidomain enzyme has a dynamic shape, permitting alternative ensembles of domains to engage the DNA in competing configurations. Conformational switching can assist in loading DNA ligase III onto nicked DNA and promoting the juxtaposition of two DNA molecules in the active site for ligation of two DNAs. EXPERIMENTAL PROCEDURES Protein Purification. LigIIIβ and ΔZnF-LigIII were purified as described previously (15), and purified protein was concentrated to 30-40 mg/mL and stored at -80 C. Selenomethioninelabeled ΔZnF-LigIII protein was expressed in BL21(DE3) using amino acids to suppress methionine biosynthesis, as described previously (19), and was purified by the same protocol as the native protein. LigIII755 (residues 1-755) and ΔZnF755 (residues 170-755) were cloned into pET28a and purified using the same protocols used for LigIIIβ and ΔZnF-LigIII, respectively. Nicked DNA Substrate Preparation. The DNA strands were synthesized on an Applied Biosystems 394 DNA/RNA Synthesizer and were desalted using a SepPak cartridge (Waters,

Cotner-Gohara et al. Inc.). The nicked DNA substrate was formed by annealing equimolar amounts of the three DNA strands [50 CGGGATGCGTddC (upstream; ddC is 20 ,30 -dideoxycytidine monophosphate), 50 PO4GTCGGACTGGC (downstream), and 50 GCCAGTCCGACGACGCATCCCG (template)] in 5 mM MES (pH 6.5) and 20 mM NaCl. Crystallization. A LigIII-DNA complex was formed by incubaton of 0.6 mM nicked DNA substrate, 0.6 mM ΔZnFLigIIIβ, 1 mM ATP, and 10 mM MgCl2. The ligase-DNA complex was mixed with an equal volume of well solution [1.8 M ammonium sulfate and 0.1 M sodium acetate (pH 5.6)]. Crystals (P41212, a = 130.1 A˚, b = 130.1 A˚, and c = 150.4 A˚) grew at 22 C by hanging drop vapor diffusion. Prior to being flashcooled in liquid nitrogen, crystals were washed in well solution and transferred to a cryoprotectant solution containing 1.8 M ammonium sulfate, 0.2 M sodium acetate (pH 5.6), and 25% glycerol. Crystals diffracted beyond 3.5 A˚ using synchrotron radiation, and there is one ΔZnF-LigIIIβ-DNA complex per asymmetric unit. X-ray Data Collection. X-ray diffraction data extending to 3.0 A˚ resolution were collected from frozen crystals at the NECAT beamline at the Advanced Photon Source (Argonne, IL) and at the MBC beamline at the Advanced Light Source (Berkeley, CA). Data from two multiwavelength anomalous dispersion experiments and one native experiment were used. X-ray data were processed using HKL2000 (20) or d*trek and then scaled using Scalepack (20, 21). Nineteen of 21 SeMet sites were located by automated Patterson searches using SOLVE (22). Heavy-atom parameters were refined, and experimental phases were calculated in SHARP using the native data set in combination with the MAD data sets (23). Experimentally phased maps had a well-defined solvent boundary and obvious electron density for both protein and nucleic acid. Phase improvement and density modification in SOLOMON in SHARP greatly enhanced the interpretability of the electron density. The binding register of the DNA with respect to the protein was determined using the clearly visible nick in the DNA backbone, and purines could be distinguished from pyrimidines. The SeMet sites, bulky amino acid side chains, and comparison to the DNA ligase I structure (14) helped define the amino acid register. The crystallographic model was constructed using COOT (24), with refinement in REFMAC (25). TLS parameters were refined using REFMAC, with the DBD, NTase, and OBD domains and DNA treated as separate domains. Figures were generated using PYMOL (www.pymol.org), and molecular surface electrostatics were calculated with APBS (26). Crystallographic data statistics are listed in Table 1. Small-Angle X-ray Scattering. LigIII was adenylated with 5 mM MgCl2 and 1 mM ATP for 1 h at 4 C, and the reaction was quenched by addition of 10 mM EDTA. LigIII was dialyzed with a buffer containing 50 mM Tris-HCl (pH 7.5), 10% glycerol, 2 mM DTT, and 250 mM NaCl. For protein-DNA complexes, 20-mer nicked DNA substrate (30 -OH nick for LigIII without ZnF and 30 -ddC nick for LigIII with ZnF) was mixed with LigIII (protein:DNA ratio of 1:1.3), and all protein-DNA complexes were purified by gel filtration. However, comparison of purified and unpurified mixtures of LigIIIβ and DNA at different molar ratios (from 1:0.8 to 1:1.2) gave comparable although not identical results. LigIIIβ binds tightly to DNA in a buffer with 50 mM Tris-HCl (pH 7.5), 10% glycerol, 2 mM DTT, and 250 mM NaCl, while ΔZnF shows salt-dependent DNA binding affinity on gel filtration, which is consistent with the optimum

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Table 1: Crystallographic Data Statisticsa SeMet-2

beamline wavelength (λ) resolution (A˚) completeness (%) redundancy Rsymc I/σ(I) phasing resolution (A˚) Rcullisd iso (cen/acen)/ano phasing powerd iso (cen/acen)/ano refinement resolution (A˚) Rcryst/Rfreee rmsd for bond lengths (A˚) rmsd for bond angles (deg)

native

SeMet-1

MADbλ1

MAD λ2

MAD λ3

NSLS 24-ID 0.9791 3.0 (3.11-3.0) 95.5 (73.5) 12.9 (5.5) 0.057 (0.602) 25.0 (2.25)

NSLS 24-ID 0.9791 3.15 (3.26-3.15) 96.7 (79.2) 5.3 (2.4) 0.094 (0.579) 11.2 (1.9)

ALS 4.2.2 0.97911 3.1 (3.21-3.1) 100 (100) 7.11 (7.24) 0.145 (0.679) 6.9 (1.9)

ALS 4.2.2 0.97935 3.11 (3.22-3.11) 99.9 (100.0) 7.12 (7.26) 0.144 (0.663) 6.9 (2.0)

ALS 4.2.2 0.96412 3.2 (3.31-3.2) 99.9 (100.0) 7.16 (7.36) 0.2 (0.7) 5.6 (1.8)

3.00 0.825/0.839/0.0721/0.679/-

3.15

3.10 -/-/0.844 -/-/1.009

3.11 0.721/0.693/0.885 0.564/0.537/0.598

3.2 0.810/0.792/0.962 0.634/0.551/0.271

3.00 0.235/0.271 0.016 1.840

a b Statistics reported in parentheses represent data for the highest-resolution P P shell. Multiwavelength anomalous dispersion (MAD) X-ray data statistics where I is the reflection intensity and ÆIæ is the average intensity of multiple from one wavelength (λ) of a three-λ MAD experiment. cRsym = |IP- ÆIæ|/ ÆIæ,P symmetry-related reflections. dAs reported by SHARP. eRcryst = |Fo - Fc|/ |Fo|, where Fo and Fc are the observed and calculated structure factor amplitudes, respectively. Rfree was calculated with a test set of reflections (5% of the data).

near 100 mM NaCl for nick joining and DNA binding activities of ΔZnF (15, 16). Thus, LigIIIβ/LigIII755-DNA complexes were purified at 250 mM NaCl to protect them from aggregation, whereas ΔZnF/ΔZnF755-DNA complexes were purified at 100 mM NaCl. SAXS data were collected at ALS beamline 12.3.1 LBNL (Berkeley, CA) (27). Incident X-rays were tuned to a wavelength (λ) of 1.0-1.5 A˚ at a sample-to-detector distance of 1.5 m, resulting in scattering vectors (q) ranging from 0.007 to 0.31 A˚-1. The scattering vector is defined as q = 4π sin θ/λ, where 2θ is the scattering angle. All experiments were performed at 20 C, and data were processed as described previously (27). Briefly, the data were acquired at short and long time exposures (0.5 and 5 s, respectively) and then scaled and merged for calculations using the entire scattering spectrum. The experimental SAXS data were evaluated for aggregation by inspection of Guinier plots (28). The radius of gyration Rg was derived by the Guinier approximation I(q) = I(0) exp(-q2Rg2/3) with the limits qRg 20 μM (15)]. The DBD of LigIII is designed to function in concert with the ZnF domain, and this domain pair binds tightly and specifically to nicked DNA (KDNA = 0.36 ( 0.06 μM) in a cooperative manner in comparison to either domain in isolation (15). In the LigIII crystal structure, the DBD packs against the core NTase and OBD domains and presumably facilitates nick binding by the catalytic core. Thus, the DBD participates in two different modes of nick recognition by LigIII: the catalytic mode of DNA end joining seen in the crystal structure and a nick-sensing mode requiring the enzyme to open so that the ZnF gains access to the DNA ends. The interaction of the LigIII DBD with DNA may coordinate the transition between different DNA binding modes that alternately engage the ZnF and catalytic core. In a comparison of the LigIII and LigI crystal structures, there are differences in the DNA contacts made by their DBDs. Differences in the amino acid sequences of the DNA-contacting loops and their interactions with DNA likely contribute to the higher binding affinity of the LigI DBD. LigI and LigIII also differ in their activities toward homopolymer substrates (33). Specifically, LigIII joins oligo(dT) molecules hybridized to poly(rA), whereas LigI does not (33). We previously proposed that the intimate contact of the LigI OBD

Cotner-Gohara et al. Table 2: Structural Parameters from SAXS Dataa experimental parameters rigid-body modeling SAXS sample

Dmax (A˚)

Rg (A˚)

LigIIIβ LigIIIβ with DNA LigIII755 LigIII755 with DNA ΔZnF ΔZnF with DNA ΔZnF755 ΔZnF755 with DNA crystal structure of the ΔZnF-DNA complex

∼194 ∼195 ∼177 ∼180 ∼167 ∼162 ∼132 ∼125 ∼103

48.5 ( 0.2 48.4 ( 0.2 45.5 ( 0.2 45.9 ( 0.2 42.9 ( 0.3 40.3 ( 0.2 36.6 ( 0.2 31.8 ( 0.1 28.5 ( 0.2

χ2 single model/MES

6.1/3.9 6.3/4.5

7.3/3.6 18.1/3.9

a Rg is the radius of gyration given by the Guinier approximation (28). Dmax is the maximum protein distance estimated from the P(r) function as shown in Figure 3. χ2 single model/MES is the goodness of fit χ2 for the best fit atomic model and multiconformational fit χ2 for MES models. In the ΔZnF755-DNA complex, χ2 for single model was calculated from the crystal structure of the ΔZnF-DNA complex.

domain with the minor groove of B-form DNA could explain discrimination against RNA-containing substrates (14), and this interaction is conserved in LigIII (Figure S4 of the Supporting Information). We therefore experimentally tested the ability of LigIII to similarly discriminate against RNA-containing polymers of heterogeneous sequence. As predicted from the crystal structure, LigIII discriminates strongly against RNA-containing heteroduplexes, failing to ligate RNA-containing heteroduplexes under conditions that are permissive for DNA ligation (Figure S4B of the Supporting Information, left and center panels). Even under less stringent conditions, at higher temperature and with 25-fold more ligase, LigIII ligates very little of the available RNA-containing substrate after 23 h (