Rapid Report pubs.acs.org/biochemistry
Open Access on 03/18/2015
Hydrogen Tunneling in a Prokaryotic Lipoxygenase Cody A. Marcus Carr†,∥ and Judith P. Klinman*,†,‡,§ †
Department of Chemistry, ‡Department of Molecular and Cell Biology, and §California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California 94720, United States S Supporting Information *
NspLOX and a single-site mutant. These studies extend the evidence for tunneling in LOX to a prokaryotic enzyme, providing a window into the impact of altered protein structure and size on hydrogen tunneling mechanisms. Kinetic Parameters for NspLOX. Reaction rates for the oxidation of h31-LA and uniformly deuterated LA are shown along with KIEs in Figure 1. The study of LA allows direct
ABSTRACT: A bacterial lipoxygenase (LOX) shows a deuterium kinetic isotope effect (KIE) that is similar in magnitude and temperature dependence to the very large KIE of eukaryotic LOXs. This occurs despite the evolutionary distance, an ∼25% smaller catalytic domain, and an increase in Ea of ∼11 kcal/mol. Site-specific mutagenesis leads to a protein variant with an Ea similar to that of the prototypic plant LOX, providing possible insight into the origin of evolutionary differences. These findings, which extend the phenomenon of hydrogen tunneling to a prokaryotic LOX, are discussed in the context of a role for protein size and/or flexibility in enzymatic hydrogen tunneling.
L
ipoxygenases (LOXs) make up a widespread family of enzymes that convert unsaturated fatty acids to allyl hydroperoxides and other oxidized products. The first step of their reaction is a net H-atom abstraction by a metal hydroxide, usually iron but occasionally manganese,1 that occurs between two double bonds to generate a radical intermediate.2 The Hatom abstraction step is of significant interest because of the large, nearly temperature-independent deuterium kinetic isotope effects (KIEs) that have been reported for enzymes from soybean3 and humans.4 The range of KIEs (30−100) is well outside the semiclassical limits and has led to models that treat the reaction as a nonadiabatic, proton-coupled electron transfer:5,6 accordingly, the proton behaves as a wave packet rather than a particle, passing from the substrate to the product via a transition state defined by thermally activated motions of the protein heavy atoms. One compelling question for LOXs, as well as other C−H bond activating enzymes, is how each protein achieves the close donor−acceptor distances needed for efficient tunneling. In particular, the overall protein size and flexibility are expected to play important roles in this phenomenon. In recent years, LOXs from cyanobacteria that have diverged significantly from their plant and animal homologues and are notably smaller in size have been described.7−9 The enzyme characterized herein from Nostoc sp. is one-half of a fusion protein that has been shown to convert α-linolenic acid to an allylic epoxide in a two-step reaction.10 In vitro assays of the C-terminal domain (residues 344−773, NspLOX) further demonstrate conversion of α-linolenic acid and linoleic acid (LA) to hydroperoxides at similar rates.8,11 In the study presented here, the expression of NspLOX in Escherichia coli is shown to yield a stable protein with ∼10% of the activity of the LOX from soybeans (SLO-1). KIEs and their temperature dependence are reported for wild-type (WT) © 2014 American Chemical Society
Figure 1. Temperature-dependent rates and KIEs for WT NspLOX and its I219A variant.
comparisons to the well-documented reactivity of LA with SLO1. We observe that the Km for NspLOX toward LA is very low [0.3−1.1 μM (Table S1 of the Supporting Information)], which, together with the characteristic lag phase seen in initial rate studies of other LOXs prevented the collection of accurate Michaelis−Menten curves at low substrate concentrations. Reaction rates were, therefore, measured under saturating conditions (ambient O2 and 10 μM LA) at all temperatures. NspLOX has a kcat at 20 °C of 19.0 ± 0.2 s−1 with h31-LA and a KIE of 118 ± 3. The Arrhenius prefactor (AH) and energy of activation (Ea) with h31-LA are quite elevated for a native LOX [AH = (2.7 ± 1.5) × 1010 s−1, and Ea(H) = 12.4 ± 0.3 kcal/mol]. The Ea(D) is slightly lower, 11.6 ± 0.3 kcal/mol, which leads to the appearance of a KIE that increases slightly with temperature; however, this difference between the isotopic Ea values is not statistically significant (P < 0.10). The KIE therefore arises solely from differences in prefactors (ΔΔS⧧). While this behavior is similar to that of SLO-1, the magnitude of ΔEa is reduced in Received: January 16, 2014 Revised: March 5, 2014 Published: March 18, 2014 2212
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Table 1. Kinetic Parameters for WT NspLOX, WT SLO-1, and Their Mutants kcat (s−1) WT NspLOXa I219A NspLOXa WT SLO-1c L546A SLO-1c
KIE
20 °C
30 °C
20 °C
30 °C
Ea(H) (kcal/mol)
ΔEa (kcal/mol)
AH (s−1)
19.0 ± 0.2 15.0 ± 0.5 275 ± 9 3.9 ± 0.3
37.1 ± 0.8b 16.7 ± 2.4b 297 ± 12 4.8 ± 0.6
118 ± 3 155 ± 7 84 ± 11 126 ± 3
128 ± 3b 164 ± 26b 81 ± 5 109 ± 4
12.4 ± 0.3 2.7 ± 0.5 1.6 ± 0.2 4.0 ± 0.7
−0.7 ± 0.3 1.1 ± 0.5 1.1 ± 0.2 1.6 ± 0.6
(2.7 ± 1.5) × 1010 (1.3 ± 1.1) × 103 (4.1 ± 1.3) × 103 (4 ± 4) × 103
a Data for WT and I219A collected at 7.5−27.5 and 5−25 °C, respectively. bExtrapolated from an Eyring fit (Figure 1) for comparison to SLO-1 at 30 °C. cFrom ref 16.
The rate constant for I219A with h31-LA at 20 °C was 15.0 ± 0.5 s−1, slightly reduced relative to that of WT NspLOX. The deuterium KIE is elevated to 155 ± 7. Unexpectedly, the magnitudes of both Ea and AH are greatly reduced relative to magnitudes of those of WT NspLOX (Table 1). Role of Protein Size and Flexibility. The characterization of I219A NspLOX provides considerable insight into the properties of the WT variant. As shown in Table 1, a smaller amino acid at position 219 in NspLOX leads to an enzyme that shows values of Ea, ΔEa, and AH almost identical to those of WT SLO-1. We propose that the reduction in the size of a large side chain within the active site of NspLOX introduces a flexibility that is absent from WT NspLOX. This increased flexibility allows the mutant enzyme to sample catalytically viable regions of the conformational landscape more easily (reducing the magnitude of Ea and AH in a concomitant manner). At the same time, the magnitude of the KIE and its temperature dependence are increased in relation to those of WT NspLOX. The latter is similar to the behavior seen most generally in mutant variants of C−H bond activating enzymes, i.e., an increase in the donor− acceptor tunneling distance that can be compensated for, in part, by a temperature-dependent search for a more suitable tunneling distance.18 The fact that WT NspLOX, with a lower kcat, is strictly speaking a less effective catalyst than SLO-1 does not necessarily mean that NspLOX is a functionally defective form of SLO-1. While the values of Ea and particularly ΔEa are highly informative from a mechanistic point of view, kcat values are not necessarily reflective of evolutionary fitness. This is especially true for enzymes whose purpose is the production of secondary metabolites, the levels of which are tightly regulated and often coupled to environmental conditions. The function of prokaryotic LOXs is not known,19 except for a report that Nostoc punctiforme produces fatty acid hydroperoxides in response to cell wounding.20 The key mechanistic property to emerge from the kinetic comparison of NspLOX and SLO-1 is the inferred change in the catalytically relevant conformational landscape. The elevated rate together with a low Ea for C−H bond activation in SLO-1 implies small conformational barriers to the achievement of tunneling ready states. By contrast, the slower rate and much higher Ea of NspLOX can be explained by a reversible trapping of the protein into noncatalytic substates. This raises the question of which changes in protein structure and flexibility may have supported a transition from NspLOX to SLO-1. Our demonstration that insertion of a single, active site mutation into NspLOX reproduces the Ea of SLO-1 suggests one pathway for conferring more conformational fitness. However, this active site alteration in NspLOX cannot improve its rate, presumably by compromising the ability of enzyme to properly constrain and/or position the H-donor and -acceptor atoms with respect to one another. The currently available sequence and structural information
relation to that of the plant enzyme (Table 1). Additional kinetic parameters are provided in Table S2 of the Supporting Information. The most striking difference between WT NspLOX and WT SLO-1 is the large enthalpy of activation for the former. This indicates that the properties that control the KIE are distinct f rom the net barrier controlling hydrogen transfer. Nagel and co-workers have recently described anomalously elevated values for Ea and AH in a thermophilic alcohol dehydrogenase operating under the nonphysiologic condition of low temperature.12 Their model invokes a reversible trapping of enzyme into inactive or reducedactivity conformational substates. The return of such molecules to the catalytically viable region of the conformational landscape requires thermally activated fluctuations that are the origin of the elevated Ea values. As an alternative, the elevated Ea for NspLOX may result from the expression of a single-domain protein, in the absence of its in vivo-partnered enzyme activity. However, it is fairly common for separate proteins to become fused within prokaryotic operons and for individual domains to gain and lose partners through the course of evolution.13 As described below, we pursued the mechanistic origin of the elevated Ea value for WT NspLOX using site-specific mutagenesis. Preparation and Characterization of I219A. NspLOX is smaller than SLO-1, lacking the N-terminal β-barrel found in most LOXs. Additionally, the catalytic domain consists of 430 amino acids in comparison to 562 for SLO-1.14 Sequences for core portions of the proteins are shown in Figure S1 of the Supporting Information, the sequences being 22% identical and 40% similar in the represented region. Despite the low degree of overall homology, we identified Ile219 in NspLOX as the analogue of the active site Leu546 in SLO-1. Leu546 sits proximal to the active site iron in SLO-1 and has been thought to position the reactive carbon of the substrate for C−H bond activation.15,16 The positions of Ile219 and Leu546 are within an 86-residue region that is relatively well conserved and seven amino acids N-terminal of a conserved isoleucine. In SLO-1, this is position 553, one helix turn away from Leu546 (Figure S2 of the Supporting Information); structures for SLO-1 mutated at position 55317 or 546 (unpublished data) indicate that the side chain positions of Leu546 and Ile553 are correlated. Thus, the choice of Ile219 as a mimic of Leu546 in SLO-1 appears wellvalidated. Analytical size-exclusion chromatography of NspLOX and I219A indicates a mixture of monomers and dimers (Figure S3 of the Supporting Information), with similar specific activities. As a control for a possible impact of enzyme concentration on specific activity, NspLOX was kinetically assayed at 20 °C across a range of protein concentrations that brackets the experimental conditions. The results (Figure S4 of the Supporting Information) indicate that the rate is linear between 3 and 264 μM enzyme. 2213
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suggest an alternate route for the tuning of catalytic fitness in SLO-1. The major structural difference between SLO-1 and NspLOX appears to lie in the size of surface loops, which are enlarged in the eukaryotic enzyme (Figure S5 and Table S3 of the Supporting Information). We suggest that a change in surface loops may have provided the catalytically strategic alteration, simultaneously ensuring a catalytically productive placement of the reactive carbon of substrate via retention of a bulky active site side chain while introducing the capability for more protein flexibility through increased surface loop size. Appropriately designed mutagenesis studies should permit further testing of this hypothesis.
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(15) Knapp, M. J., Rickert, K., and Klinman, J. P. (2002) J. Am. Chem. Soc. 124, 3865−3874. (16) Rickert, K., and Klinman, J. P. (1999) Biochemistry 38, 12218− 12228. (17) Meyer, M. P., Tomchick, D. R., and Klinman, J. P. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 1146−1151. (18) Nagel, Z. D., and Klinman, J. P. (2009) Nat. Chem. Biol. 5, 543− 550. (19) Hansen, J., Garreta, A., Benincasa, M., Fusté, M. C., Busquets, M., and Manresa, A. (2013) Appl. Microbiol. Biotechnol. 97, 4737−4747. (20) Lang, I., and Feussner, I. (2007) Phytochemistry 68, 1120−1127.
ASSOCIATED CONTENT
* Supporting Information S
Experimental methods, detailed kinetics data, and data from controls. This material is available free of charge via the Internet at http://pubs.acs.org.
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AUTHOR INFORMATION
Corresponding Author
*E-mail:
[email protected]. Phone: (510) 642-2668. Present Address ∥
C.A.M.C.: Kiverdi, Inc., 2929 7th St., Berkeley, CA 94710.
Funding
Supported by National Institutes of Health Grants GM025765 to J.P.K. and T32 GM008295 to C.A.M.C. Notes
The authors declare no competing financial interest.
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ACKNOWLEDGMENTS We thank Alan R. Brash for providing the NspLOX gene. REFERENCES
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