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Hypocholesterolemic Activity of Curcumin is Mediated by DownRegulating the Expression of Niemann-Pick C1-Like 1 in Hamsters Dan Feng, Jun Zou, Shanshan Zhang, Xuechun Li, and Minqi Lu J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b04102 • Publication Date (Web): 21 Dec 2016 Downloaded from http://pubs.acs.org on December 22, 2016
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Journal of Agricultural and Food Chemistry
Hypocholesterolemic Activity of Curcumin is Mediated by Down-Regulating the Expression of Niemann-Pick C1-Like 1 in Hamsters
Running title: Curcumin elicits hypocholesterolemic effects by inhibiting NPC1L1
Dan Feng*,†, Jun Zou‡, Shanshan Zhang†, Xuechun Li† and Minqi Lu†
Affiliation †
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Preventive Medicine, School of Public Health, Sun Yat-sen University, 510080, China
‡
Department of Cardiology, Affiliated NanHai Hospital of Southern Medical University, 528200, China
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT: We previously demonstrated that curcumin reduces cholesterol
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absorption in Caco-2 cells through down-regulating NPC1L1 expression, but the in
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vivo effect of curcumin on intestinal cholesterol absorption remains unknown. The
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present study was aimed to investigate the effects and mechanisms of curcumin
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consumption on cholesterol absorption in hamsters. Male hamsters were fed a
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high-fat diet supplemented with or without curcumin (0.05% w/w) for 12 weeks.
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Curcumin supplementation significantly decreased serum total cholesterol
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(TC)( 6.86 ±0.27 to 3.50 ±0.24 mmol/L), triglyceride (TG) (5.07 ±0.34 to 3.72 ±
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0.40 mmol/L), and low density lipoprotein cholesterol ( 2.58 ±0.19 to 1.71 ±0.15
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mmol/L) levels as well as liver TC (11.6 ±0.05 to 7.2 ±0.03 mg/g) and TG (30.3 ±
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0.22 to 25.2 ±0.18 mg/g) levels (P
