Anal. Chem. 2003, 75, 3429-3434
Imaging of Membrane Lipids in Single Cells by Imprint-Imaging Time-of-Flight Secondary Ion Mass Spectrometry Peter Sjo 1 vall,*,† Jukka Lausmaa,† Håkan Nygren,‡ Lennart Carlsson,† and Per Malmberg‡
Chemistry and Materials Technology, SP Swedish National Testing and Research Institute, P.O. Box 857, SE-501 15 Borås, Sweden, and Department of Anatomy and Cell Biology, Go¨teborg University, P.O. Box 420, SE-405 30 Go¨teborg, Sweden
A new method for identification and localization of organic molecules in biological samples is described. The method involves making an imprint of a biological sample on a silver (Ag) surface and subsequent analysis of the imprint by imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS). Using this method, detection of unfragmented, Ag cationized molecules at a spatial resolution of
