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Biophysical Chemistry, Biomolecules, and Biomaterials; Surfactants and Membranes
Impacts of Lipase Enzyme on the Surface Properties of Marine Aerosols Jamie M. Schiffer, Man Luo, Abigail C. Dommer, Gray Thoron, Matthew A. Pendergraft, Mitchell V. Santander, Dolan Lucero, Emilia Pecora de Barros, Kimberly Ann Prather, Vicki H. Grassian, and Rommie E. Amaro J. Phys. Chem. Lett., Just Accepted Manuscript • DOI: 10.1021/acs.jpclett.8b01363 • Publication Date (Web): 19 Jun 2018 Downloaded from http://pubs.acs.org on June 22, 2018
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The Journal of Physical Chemistry Letters
Impacts of Lipase Enzyme on the Surface Properties of Marine Aerosols J. M. Schiffer,1 M. Luo,1 A. C. Dommer,1 G. Thoron,2 M. Pendergraft, 3 M.V. Santander,1 D. Lucero,3 E. P. Barros, 1 K. A. Prather,1,3 V.H. Grassian,1,3,4* R. E. Amaro,1* Author Affiliations 1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0378, United States. 2. San Diego Met High School, 7250 Mesa College Drive, Room K203, San Diego, California 92111, United States 3. Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093, U.S.A. 4. Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0378, United States.
Corresponding Authors:
* Rommie E. Amaro;
[email protected] * Vicki H. Grassian:
[email protected] 1
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Abstract Triacylglycerol lipases have recently been shown to be transferred from the ocean to the atmosphere in atmospheric sea spray aerosol (SSA). Lipases have the potential to alter the composition of SSA; however, the structure and properties of enzymes in the high salt, high ionic strength and low pH conditions found in SSA have never been explored. Here, we study the dynamics of Burkholderia cepacia triacylglycerol lipase (BCL) at SSA model surfaces comprised of palmitic acid and dipalmitoylphosphatidic acid (DPPA), two commonly found lipids at SSA surfaces. Surface adsorption Langmuir isotherm experiments and all-atom explicit solvent molecular dynamics simulations together illuminate how and why BCL expands the ordering of lipids at palmitic acid surfaces the most at pH
