Anal. Chem. 1996, 68, 3274-3276
Improved Accuracy and Precision in the Determination of Association Constants Shirley M. Hoenigman and Christine E. Evans*
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055
Association constants for inclusion complexes are commonly determined by varying the analytical concentration of the “host” species while maintaining the analytical concentration of “guest” species constant. In implementing the experiment, commonly referred to as BenesiHildebrand analysis, conditions are controlled such that all necessary concentration requirements are fulfilled and the guest molecule in the unbound state does not contribute appreciably to the overall signal (
