in ac research
In AC Research contains brief introductions to the research articles appearing in the January 1 issue. A free updated table of contents is available on the Web (http://pubs.acs.org/ac).
SPR of DNA hybridization adsorption. Robert Corn and colleagues at the University of Wisconsin– Madison use surface plasmon resonance (SPR) imaging to quantitatively detect the hybridization adsorption of short unlabeled DNA oligonucleotides at low concentration and, for the first time, the hybridization adsorption of unlabeled RNA oligonucleotides from E. coli onto a DNA array. (“Surface Plasmon Resonance Imaging Measurements of DNA and RNA Hybridization Adsorption onto DNA Microarrays”; 10.1021/ac0010431; p 1)
Peptide and protein mapping. Electron capture dissociation has the advantage of being able to cleave between a high proportion of amino acids without losing posttranslational modifications. Mark E. Hemling, Steven A. Carr, Fred McLafferty, and colleagues at SmithKline Beecham Pharmaceuticals and Cornell University extend this capability to phosphorylation. (“Phosphopeptide/Phosphoprotein Mapping by Electron Capture Dissociation Mass Spectrometry”; 10.1021/ac000703z; p 19) Mixed-lipid films for detecting toxins. Gertrud Puu at the Defence Research Establishment (Sweden) describes the use of thin films of lipid mixtures deposited on the surface of an optical evanescent biosensor’s cuvette to detect toxins. (“An Approach for Analysis of Protein Toxins Based on Thin Films of Lipid Mixtures in an Optical Biosensor”; 10.1021/ac000619j; p 72) Analyte bound to be detected. Itamar Willner and co-workers at the Hebrew University of Jerusalem (Israel) and Sandia National Laboratories enhance the sensing of antigen– antibody interactions and oligonucleotide–DNA binding with the formation of a micromembrane on gold electrodes. (“Liposomes Labeled with Biotin and Horseradish Peroxidase: A Probe for the Enhanced Amplification of Antigen–Antibody or Oligonucleotide–DNA Sensing Processes by the Precipitation of an Insoluble Product on Electrodes”; 10.1021/ac000819v; p 91)
Single cell analysis. To enhance the analysis of single cells, Sheri Lillard and Shujun Chen at the University of California–Riverside describe new CE instrumentation that features continuous cell introduction. They report analysis times of
