In Vivo–in Vitro and XANES Spectroscopy ... - ACS Publications

Jun 28, 2011 - Ruby et al.12 (PBET method and a SpragueАDawley rat model) ..... Lead to Juvenile Swine Dosed with Soil from the Smuggler Mountain...
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In Vivo in Vitro and XANES Spectroscopy Assessments of Lead Bioavailability in Contaminated Periurban Soils Euan Smith,*,†,‡ Ivan M. Kempson,§ Albert L. Juhasz,†,‡ John Weber,†,‡ Allan Rofe,|| Dorota Gancarz,|| Ravi Naidu,†,‡ Ronald G. McLaren,^ and Markus Gr€afe# †

Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, SA 5095, Australia Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Mawson Lakes, SA 5095, Australia. § Institute of Physics, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan Veterinary Services Division, Institute of Medical and Veterinary Science, Gilles Plains, SA 5086, Australia ^ Faculty of Agriculture and Life Sciences, Lincoln University, Christchurch, New Zealand # CSIRO-PS&E, Australian Minerals Research Centre, 7 Conlon Street, Waterford, WA 6152, Australia

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bS Supporting Information ABSTRACT: Lead (Pb) bioaccessibility was assessed using 2 in vitro methods in 12 Pb-contaminated soils and compared to relative Pb bioavailability using an in vivo mouse model. In vitro Pb bioaccessibility, determined using the intestinal phase of the Solubility Bioaccessibility Research Consortium (SBRC) assay, strongly correlated with in vivo relative Pb bioavailability (R2 = 0.88) following adjustment of Pb dissolution in the intestinal phase with the solubility of Pb acetate at pH 6.5 (i.e., relative Pb bioaccessibility). A strong correlation (R2 = 0.78) was also observed for the relative bioaccessibility leaching procedure (RBALP), although the method overpredicted in vivo relative Pb bioavailability for soils where values were